The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase

  • Authors:
    • Rhiannon M. Owen
    • Rachael D. Baker
    • Scott Bader
    • Malcolm G. Dunlop
    • Iain D. Nicholl
  • View Affiliations

  • Published online on: January 1, 2007     https://doi.org/10.3892/or.17.1.111
  • Pages: 111-116
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

ethyl-CpG binding protein 4 (MBD4) is a mismatch-specific G:T and G:U DNA glycosylase. During an analysis of MBD4 expression in HeLa cells we noted the presence of an unexpectedly short reverse transcribed product. This cDNA lacked the region encoding the methyl-binding domain and exon 3 of MBD4 but retained the glycosylase domain. Sequence comparison indicates the existence of a previously unreported cryptic splice site in the MBD4 genomic sequence thus illuminating a mechanism whereby a glycosylase acquired a methyl-binding capacity, thus targeting potential mutagenic CpG sites. In vitro assays of this highly purified species, refolded in arginine rich conditions, confirmed that this unique, short version of MBD4 possessed uracil DNA glycosylase but not thymine DNA glycosylase activity. We conclude that the identification of a transcript encoding a short version of MBD4 indicates that MBD4 expression may be more complex than previously reported, and is worthy of further investigation.

Related Articles

Journal Cover

January 2007
Volume 17 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Owen RM, Baker RD, Bader S, Dunlop MG and Nicholl ID: The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase. Oncol Rep 17: 111-116, 2007.
APA
Owen, R.M., Baker, R.D., Bader, S., Dunlop, M.G., & Nicholl, I.D. (2007). The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase. Oncology Reports, 17, 111-116. https://doi.org/10.3892/or.17.1.111
MLA
Owen, R. M., Baker, R. D., Bader, S., Dunlop, M. G., Nicholl, I. D."The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase". Oncology Reports 17.1 (2007): 111-116.
Chicago
Owen, R. M., Baker, R. D., Bader, S., Dunlop, M. G., Nicholl, I. D."The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase". Oncology Reports 17, no. 1 (2007): 111-116. https://doi.org/10.3892/or.17.1.111