Mutational analysis of Chk1, Chk2, Apaf1 and Rb1 in human malignant melanoma cell lines

  • Authors:
    • Thilo Papp
    • Annett Niemetz
    • Nils Dosdahl
    • Krishan Kumar
    • Dietmar Schiffmann
  • View Affiliations

  • Published online on: January 1, 2007     https://doi.org/10.3892/or.17.1.135
  • Pages: 135-140
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Abstract

Four tumour suppressor genes (Chk1, Chk2, Apaf1 and Rb1) in nine human malignant melanoma cell lines were screened for a loss of gene expression, point mutations and small deletions/insertions by cDNA-based DGGE/SCCP analysis. In two cell lines alterations of the investigated genes could be demonstrated. This result confirms our assumption of the participation of dysfunctional p53 inducer/effector genes in human melanoma aetiology. Furthermore, it points towards the probable principal role of diverse alternative p53-pathway disruption mechanisms in this highly therapy-resistant malignancy without affecting p53 itself. To our knowledge, this is the first CHK1/CHK2 mutation screening in human melanoma.

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January 2007
Volume 17 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Papp T, Niemetz A, Dosdahl N, Kumar K and Schiffmann D: Mutational analysis of Chk1, Chk2, Apaf1 and Rb1 in human malignant melanoma cell lines. Oncol Rep 17: 135-140, 2007.
APA
Papp, T., Niemetz, A., Dosdahl, N., Kumar, K., & Schiffmann, D. (2007). Mutational analysis of Chk1, Chk2, Apaf1 and Rb1 in human malignant melanoma cell lines. Oncology Reports, 17, 135-140. https://doi.org/10.3892/or.17.1.135
MLA
Papp, T., Niemetz, A., Dosdahl, N., Kumar, K., Schiffmann, D."Mutational analysis of Chk1, Chk2, Apaf1 and Rb1 in human malignant melanoma cell lines". Oncology Reports 17.1 (2007): 135-140.
Chicago
Papp, T., Niemetz, A., Dosdahl, N., Kumar, K., Schiffmann, D."Mutational analysis of Chk1, Chk2, Apaf1 and Rb1 in human malignant melanoma cell lines". Oncology Reports 17, no. 1 (2007): 135-140. https://doi.org/10.3892/or.17.1.135