Induction of apoptosis in an estrogen-responsive mouse Leydig tumor cell by leukotriene

  • Authors:
    • H. G. Goto
    • Y. Nishizawa
    • H. Katayama
    • T. Murashima
    • M. Yamasaki
    • Y. Tanigaki
    • S. Kimura
    • S. Fushiki
  • View Affiliations

  • Published online on: January 1, 2007     https://doi.org/10.3892/or.17.1.225
  • Pages: 225-232
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Abstract

For estrogen-responsive B-1F cells, established from estrogen-responsive mouse Leydig cell tumor, it has been reported that the 5-lipoxygenase (5-LOX) metabolic pathway appears to be associated with cell growth. The addition of 5-LOX inhibitor 2-(12-hydroxydodeca-5,10-diyl)-3,5,6-trimethyl-1,4-benzoquinone (AA861) to the medium resulted in a dose-dependent increase in cell yield as described previously. When the growth of the palpable tumors was measured, AA861 had stimulated in vivo tumor growth in adult male mouse inoculated B-1F cells. The effects of AA861 and 17β-estradiol (E2) on the contents of various arachidonic acid metabolites in B-1F cells and their conditioned medium were examined. Although AA861 and E2 decreased the contents of leukotrienes (LTs), the two did not significantly change those of prostaglandins, thromboxan, prostacyclin, 12-hydroxyeicosatetraenoic acid (HETE) and 15-HETE. In immunohistochemical study B-1F cells show positive staining for 5-LOX in the E2-depleted condition, while E2 decreased the expression of 5-LOX. The decrease of the intensities of 79-kDa 5-LOX protein and 403-bp RT-PCR product bands was observed. The growth of Morpholino-anti oligo delivered B-1F cells was higher than that of Standard control oligo delivered cells. The delivery of Morpholino-anti oligo into B-1F cells caused the decrease of contents of LTs and 5-HETE in the cells and medium, and the reduction of 5-LOX activity. When LTD4 was added in the culture medium, the increasing concentrations of LTD4 resulted in a significant inhibition of cell yields of E2-treated B-1F cells. Morphological changes such as nuclear condensation and fragmentation, and DNA ladder pattern were demonstrated in E2-stimulated B-1F cells treated with LTD4 as well as in control cells cultured in the basal medium. These results implicate that 5-LOX at least plays an important role in the growth of B-1F cells and LD4 induces the apoptosis of B-1F cells.

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January 2007
Volume 17 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Goto HG, Nishizawa Y, Katayama H, Murashima T, Yamasaki M, Tanigaki Y, Kimura S and Fushiki S: Induction of apoptosis in an estrogen-responsive mouse Leydig tumor cell by leukotriene. Oncol Rep 17: 225-232, 2007
APA
Goto, H.G., Nishizawa, Y., Katayama, H., Murashima, T., Yamasaki, M., Tanigaki, Y. ... Fushiki, S. (2007). Induction of apoptosis in an estrogen-responsive mouse Leydig tumor cell by leukotriene. Oncology Reports, 17, 225-232. https://doi.org/10.3892/or.17.1.225
MLA
Goto, H. G., Nishizawa, Y., Katayama, H., Murashima, T., Yamasaki, M., Tanigaki, Y., Kimura, S., Fushiki, S."Induction of apoptosis in an estrogen-responsive mouse Leydig tumor cell by leukotriene". Oncology Reports 17.1 (2007): 225-232.
Chicago
Goto, H. G., Nishizawa, Y., Katayama, H., Murashima, T., Yamasaki, M., Tanigaki, Y., Kimura, S., Fushiki, S."Induction of apoptosis in an estrogen-responsive mouse Leydig tumor cell by leukotriene". Oncology Reports 17, no. 1 (2007): 225-232. https://doi.org/10.3892/or.17.1.225