Dietary β-cryptoxanthin inhibits N-butyl-N-(4-hydroxybutyl)nitrosamine-induced urinary bladder carcinogenesis in male ICR mice

  • Authors:
    • Katsuhito Miyazawa
    • Shingo Miyamoto
    • Rikako Suzuki
    • Yumiko Yasui
    • Ryosuke Ikeda
    • Hiroyuki Kohno
    • Masamichi Yano
    • Takuji Tanaka
    • Kazuya Hata
    • Koji Suzuki
  • View Affiliations

  • Published online on: February 1, 2007     https://doi.org/10.3892/or.17.2.297
  • Pages: 297-304
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Recent epidemiological studies have indicated that high dietary consumption of fruit and vegetables results in lower risk of bladder cancer. To confirm these findings, we investigated in the current study the effects of dietary administration with β-cryptoxanthin extracted from Citras unshiu oranges on N-butyl-N-(4-hydroxybutyl)nitrosamine (OH-BBN)-induced urinary bladder carcinogenesis in mice. Male ICR mice were divided into 6 experimental and control groups. Groups 1 through 4 were given OH-BBN (500 ppm) in drinking water for 6 weeks to induced urinary bladder neoplasms. Mice in groups 2, 3 and 4 were fed the diets mixed with 1, 5 and 25 ppm of β-cryptoxanthin, respectively, starting 1 week after the cessation of OH-BBN exposure, and kept on these diets for 24 weeks until the termination of the study. Group 5 was treated with the diet containing the test compound (25 ppm) alone, and group 6 served as an untreated control. All animals were sacrificed at week 32 for histopathology and immunohistochemistry (cyclin D1). Feeding with β-cryptoxanthin decreased the incidence and multiplicity of preneoplastic and neoplastic lesions of urinary bladder. Notably, the highest dose (25 ppm) of the test chemical significantly lowered the occurrence of bladder carcinoma, in conjunction with reducing the cyclin D1-positive cell ratio. These findings suggest that β-cryptoxanthin is able to prevent OH-BBN-induced bladder carcinogenesis in mice.

Related Articles

Journal Cover

February 2007
Volume 17 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Miyazawa K, Miyamoto S, Suzuki R, Yasui Y, Ikeda R, Kohno H, Yano M, Tanaka T, Hata K, Suzuki K, Suzuki K, et al: Dietary β-cryptoxanthin inhibits N-butyl-N-(4-hydroxybutyl)nitrosamine-induced urinary bladder carcinogenesis in male ICR mice. Oncol Rep 17: 297-304, 2007.
APA
Miyazawa, K., Miyamoto, S., Suzuki, R., Yasui, Y., Ikeda, R., Kohno, H. ... Suzuki, K. (2007). Dietary β-cryptoxanthin inhibits N-butyl-N-(4-hydroxybutyl)nitrosamine-induced urinary bladder carcinogenesis in male ICR mice. Oncology Reports, 17, 297-304. https://doi.org/10.3892/or.17.2.297
MLA
Miyazawa, K., Miyamoto, S., Suzuki, R., Yasui, Y., Ikeda, R., Kohno, H., Yano, M., Tanaka, T., Hata, K., Suzuki, K."Dietary β-cryptoxanthin inhibits N-butyl-N-(4-hydroxybutyl)nitrosamine-induced urinary bladder carcinogenesis in male ICR mice". Oncology Reports 17.2 (2007): 297-304.
Chicago
Miyazawa, K., Miyamoto, S., Suzuki, R., Yasui, Y., Ikeda, R., Kohno, H., Yano, M., Tanaka, T., Hata, K., Suzuki, K."Dietary β-cryptoxanthin inhibits N-butyl-N-(4-hydroxybutyl)nitrosamine-induced urinary bladder carcinogenesis in male ICR mice". Oncology Reports 17, no. 2 (2007): 297-304. https://doi.org/10.3892/or.17.2.297