EGFRvIII mutation in lung cancer correlates with increased EGFR copy number

  • Authors:
    • Hidefumi Sasaki
    • Osamu Kawano
    • Katsuhiko Endo
    • Haruhiro Yukiue
    • Motoki Yano
    • Yoshitaka Fujii
  • View Affiliations

  • Published online on: February 1, 2007     https://doi.org/10.3892/or.17.2.319
  • Pages: 319-323
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Abstract

Overexpression of the epidermal growth factor receptor (EGFR) is caused by EGFR gene amplification and is sometimes associated with expression of a variant EGFR (deletion exon 2-7 or EGFRvIII). EGFRvIII mutation has oncogenic potential and is investigated as a potential therapeutic target. We genotyped the EGFRvIII mutation status in 252 surgically treated lung cancer cases. The presence or absence of EGFRvIII mutation was analyzed by real-time quantitative polymerase chain reaction (PCR) with mutation specific sensor and anchor probes. EGFR copy number was evaluated with PCR-based assay. EGFR mutation status at kinase domain has been examined and reported. EGFRvIII mutation was found on 8 of 252 patients. All patients were male, smokers, and 7 had squamous cell carcinoma. The mutation status was significantly correlated with pathological subtypes (squamous cell carcinoma vs. adenocarcinoma, p=0.0114). Sixty EGFR mutations at kinase domain exclusively existed with EGFRvIII mutations. EGFR gene copy number was significantly higher in EGFRvIII mutant (4.711plusmn;4.968) than in non-EGFRvIII mutant (2.284plusmn;1.224) (p=0.0001). EGFRvIII gene mutation might be one of the mechanisms of increased EGFR copy number. Further studies are needed to confirm the mechanisms of EGFRvIII mutations for possible anti-EGFR therapy for lung cancer.

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February 2007
Volume 17 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Sasaki H, Kawano O, Endo K, Yukiue H, Yano M and Fujii Y: EGFRvIII mutation in lung cancer correlates with increased EGFR copy number. Oncol Rep 17: 319-323, 2007
APA
Sasaki, H., Kawano, O., Endo, K., Yukiue, H., Yano, M., & Fujii, Y. (2007). EGFRvIII mutation in lung cancer correlates with increased EGFR copy number. Oncology Reports, 17, 319-323. https://doi.org/10.3892/or.17.2.319
MLA
Sasaki, H., Kawano, O., Endo, K., Yukiue, H., Yano, M., Fujii, Y."EGFRvIII mutation in lung cancer correlates with increased EGFR copy number". Oncology Reports 17.2 (2007): 319-323.
Chicago
Sasaki, H., Kawano, O., Endo, K., Yukiue, H., Yano, M., Fujii, Y."EGFRvIII mutation in lung cancer correlates with increased EGFR copy number". Oncology Reports 17, no. 2 (2007): 319-323. https://doi.org/10.3892/or.17.2.319