Establishment and characterization of a human adenoid cystic carcinoma cell line forming colonies cultured in collagen gel and transplantable in nude mice

  • Authors:
    • Noriaki Tanaka
    • Kazuhiko Urabe
    • Susumu Hashitani
    • Kazunari Sakurai
    • Masahiro Urade
  • View Affiliations

  • Published online on: February 1, 2007     https://doi.org/10.3892/or.17.2.335
  • Pages: 335-340
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We established a new cell line (ACCNS) from human adenoid cystic carcinoma (AdCC) of the maxilla, using tissue culture techniques and it has been successfully subcultured for more than 100 passages during 2 years. The population doubling time was approximately 39.2 h on a plastic dish. In type I collagen gel culture, the cells formed spherical colonies by day 7 after seeding. The colonies showed tubular and solid structures, and eosinophilic material stained with mucicarmine was revealed in the inner space. Immunohistochemically, ACCNS cells demonstrated expressions of keratin, α-smooth muscle actin, vimentin and S-100 protein, similar to those of original AdCC. These findings indicate that ACCNS cells possess the characteristics of AdCC. In addition, inoculation of 4.0x106 cells into nude mice developed tumors that were histologically confirmed as undifferentiated carcinoma. Therefore, ACCNS is the first AdCC cell line with tumorigenicity in nude mice. Based on these results, ACCNS provides a useful culture model of AdCC to analyze the biological characteristics and behavior of this tumor.

Related Articles

Journal Cover

February 2007
Volume 17 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tanaka N, Urabe K, Hashitani S, Sakurai K and Urade M: Establishment and characterization of a human adenoid cystic carcinoma cell line forming colonies cultured in collagen gel and transplantable in nude mice. Oncol Rep 17: 335-340, 2007
APA
Tanaka, N., Urabe, K., Hashitani, S., Sakurai, K., & Urade, M. (2007). Establishment and characterization of a human adenoid cystic carcinoma cell line forming colonies cultured in collagen gel and transplantable in nude mice. Oncology Reports, 17, 335-340. https://doi.org/10.3892/or.17.2.335
MLA
Tanaka, N., Urabe, K., Hashitani, S., Sakurai, K., Urade, M."Establishment and characterization of a human adenoid cystic carcinoma cell line forming colonies cultured in collagen gel and transplantable in nude mice". Oncology Reports 17.2 (2007): 335-340.
Chicago
Tanaka, N., Urabe, K., Hashitani, S., Sakurai, K., Urade, M."Establishment and characterization of a human adenoid cystic carcinoma cell line forming colonies cultured in collagen gel and transplantable in nude mice". Oncology Reports 17, no. 2 (2007): 335-340. https://doi.org/10.3892/or.17.2.335