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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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April 2007 Volume 17 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Diagnostic utility of dual fusion PML/RARα translocation DNA probe (D-FISH) in acute promyelocytic leukemia

  • Authors:
    • Thomas S.K. Wan
    • Chi-Chiu So
    • Koon-Chun Hui
    • Sze-Fai Yip
    • Edmond S.K. Ma
    • Li-Chong Chan

  • View Affiliations / Copyright

    Affiliations: Hematology Division, Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, P.R. China. wantsk@hkucc.hku.hk
  • Pages: 799-805
    |
    Published online on: April 1, 2007
       https://doi.org/10.3892/or.17.4.799
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Abstract

Translocation(15;17) leading to the formation of fusion gene PML/RARα is the diagnostic hallmark of acute promyelocytic leukemia (APL). Interphase fluorescence in situ hybridization (FISH) is one of the diagnostic tools employed for the detection of PML/RARα rearrangement. Using a dual color dual fusion (D-FISH) PML/RARα translocation DNA probe which hybridises both to PML/RARα and RARα/PML fusion genes, we characterised the FISH pattern of 52 APL patients at diagnosis and correlated the findings with conventional cytogenetics and RT-PCR analysis. The diagnostic sensitivity of the probe for PML/RARα was 100%. Seven patients had atypical D-FISH patterns; two had a masked PML/RARα fusion signal caused by the insertion of PML into RARα on 17q; 3 had an extra copy of PML/RARα in the form of isochromosome der(17)(q10)t(15;17) and one had duplication of the normal RARα gene with an ider(17q) masquerading as i(17)(q10). There was also one case of t(7;17;15) with a typical D-FISH pattern and in which metaphase FISH suggested an unusual 4-point break. In summary, PML/RARα D-FISH is a highly sensitive method for confirming diagnosis of APL. However D-FISH cannot be solely relied on for the diagnosis of APL owing to atypical patterns which are infrequently observed in cases with additional 17q structural abnormalities, gene insertion and gene duplication.

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Copy and paste a formatted citation
Spandidos Publications style
Wan TS, So C, Hui K, Yip S, Ma ES and Chan L: Diagnostic utility of dual fusion PML/RARα translocation DNA probe (D-FISH) in acute promyelocytic leukemia. Oncol Rep 17: 799-805, 2007.
APA
Wan, T.S., So, C., Hui, K., Yip, S., Ma, E.S., & Chan, L. (2007). Diagnostic utility of dual fusion PML/RARα translocation DNA probe (D-FISH) in acute promyelocytic leukemia. Oncology Reports, 17, 799-805. https://doi.org/10.3892/or.17.4.799
MLA
Wan, T. S., So, C., Hui, K., Yip, S., Ma, E. S., Chan, L."Diagnostic utility of dual fusion PML/RARα translocation DNA probe (D-FISH) in acute promyelocytic leukemia". Oncology Reports 17.4 (2007): 799-805.
Chicago
Wan, T. S., So, C., Hui, K., Yip, S., Ma, E. S., Chan, L."Diagnostic utility of dual fusion PML/RARα translocation DNA probe (D-FISH) in acute promyelocytic leukemia". Oncology Reports 17, no. 4 (2007): 799-805. https://doi.org/10.3892/or.17.4.799
Copy and paste a formatted citation
x
Spandidos Publications style
Wan TS, So C, Hui K, Yip S, Ma ES and Chan L: Diagnostic utility of dual fusion PML/RARα translocation DNA probe (D-FISH) in acute promyelocytic leukemia. Oncol Rep 17: 799-805, 2007.
APA
Wan, T.S., So, C., Hui, K., Yip, S., Ma, E.S., & Chan, L. (2007). Diagnostic utility of dual fusion PML/RARα translocation DNA probe (D-FISH) in acute promyelocytic leukemia. Oncology Reports, 17, 799-805. https://doi.org/10.3892/or.17.4.799
MLA
Wan, T. S., So, C., Hui, K., Yip, S., Ma, E. S., Chan, L."Diagnostic utility of dual fusion PML/RARα translocation DNA probe (D-FISH) in acute promyelocytic leukemia". Oncology Reports 17.4 (2007): 799-805.
Chicago
Wan, T. S., So, C., Hui, K., Yip, S., Ma, E. S., Chan, L."Diagnostic utility of dual fusion PML/RARα translocation DNA probe (D-FISH) in acute promyelocytic leukemia". Oncology Reports 17, no. 4 (2007): 799-805. https://doi.org/10.3892/or.17.4.799
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