Expression of cyclin A in human leukemia cell line HL-60 following treatment with doxorubicin and etoposide: The potential involvement of cyclin A in apoptosis

  • Authors:
    • Agnieszka Zuryn
    • Alina Grzanka
    • Aleksandra Stepien
    • Dariusz Grzanka
    • Robert Debski
    • Dariusz Smolinski
  • View Affiliations

  • Published online on: May 1, 2007     https://doi.org/10.3892/or.17.5.1013
  • Pages: 1013-1019
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Abstract

We investigated expression of cyclin A in HL-60 cells after induction of apoptosis with doxorubicin and etoposide. Following apoptotic trigger, both cells arrested in G2/M phase of the cell cycle and changes in morphology were noticed. Moreover, compared to control, the number of cells with cyclin A expression was changed and translocation of this protein from the nucleus to the cytoplasm was observed. The decrease in the number of cells with cyclin A expression, followed by the increase, and cyclin A distribution throughout the cell, appeared to be dose-dependent. Cells treated with lower doses of doxorubicin and etoposide as well as the untreated cells were found to have cyclin A scattered mainly throughout the nucleus. However, immunogold labeling of cyclin A in both cell lines treated with 5- and 10-µM doses of doxorubicin, and 20 and 200 µM of etoposide was observed more often in the cytoplasm than in the nucleus. Cells with features of apoptosis with bodies resembling micro-nuclei labeled with gold particles for cyclin A were recognized. However, the small amount of giant cells was also seen. These results suggest that cyclin A expression is linked to cell death pathways.

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May 2007
Volume 17 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zuryn A, Grzanka A, Stepien A, Grzanka D, Debski R and Smolinski D: Expression of cyclin A in human leukemia cell line HL-60 following treatment with doxorubicin and etoposide: The potential involvement of cyclin A in apoptosis. Oncol Rep 17: 1013-1019, 2007
APA
Zuryn, A., Grzanka, A., Stepien, A., Grzanka, D., Debski, R., & Smolinski, D. (2007). Expression of cyclin A in human leukemia cell line HL-60 following treatment with doxorubicin and etoposide: The potential involvement of cyclin A in apoptosis. Oncology Reports, 17, 1013-1019. https://doi.org/10.3892/or.17.5.1013
MLA
Zuryn, A., Grzanka, A., Stepien, A., Grzanka, D., Debski, R., Smolinski, D."Expression of cyclin A in human leukemia cell line HL-60 following treatment with doxorubicin and etoposide: The potential involvement of cyclin A in apoptosis". Oncology Reports 17.5 (2007): 1013-1019.
Chicago
Zuryn, A., Grzanka, A., Stepien, A., Grzanka, D., Debski, R., Smolinski, D."Expression of cyclin A in human leukemia cell line HL-60 following treatment with doxorubicin and etoposide: The potential involvement of cyclin A in apoptosis". Oncology Reports 17, no. 5 (2007): 1013-1019. https://doi.org/10.3892/or.17.5.1013