Amplification and overexpression of Aurora-A in esophageal squamous cell carcinoma

  • Authors:
    • Shang-Bin Yang
    • Xiao-Bo Zhou
    • Hong-Xia Zhu
    • Lan-Ping Quan
    • Jin-Feng Bai
    • Jie He
    • Yan-Ning Gao
    • Shu-Jun Cheng
    • Ning-Zhi Xu
  • View Affiliations

  • Published online on: May 1, 2007     https://doi.org/10.3892/or.17.5.1083
  • Pages:1083-1088
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Aurora-A/BTAK/STK15 gene which encodes a centrosome-associated kinase is located on chromosome 20q13.2, a highly amplified region in various human tumors. Recent studies have demonstrated the overexpression and amplification of Aurora-A in many malignant human cancers. The purpose of this study was to investigate the amplification and expression of Aurora-A in esophageal squamous cell carcinoma. Amplification of Aurora-A was determined by fluorescence in situ hybridization in 7 esophageal cancer cell lines and real-time PCR in 29 esophageal cancer samples. We detected Aurora-A expression in 7 esophageal cancer cell lines and 38 esophageal cancers samples by semi-quantitative reverse transcription-PCR and Western blot hybridization. The amplification of Aurora-A was detected in 27 of 29 (93.1%) esophageal cancer samples and 6 of 7 (85.7%) cancer cell lines. Aurora-A was overexpressed in 27 of 38 (71.1%) esophageal cancer samples and all 7 esophageal cancer cell lines. We conclude that Aurora-A is amplified and overexpressed in esophageal squamous cancer.

Related Articles

Journal Cover

May 2007
Volume 17 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Yang, S., Zhou, X., Zhu, H., Quan, L., Bai, J., He, J. ... Xu, N. (2007). Amplification and overexpression of Aurora-A in esophageal squamous cell carcinoma. Oncology Reports, 17, 1083-1088. https://doi.org/10.3892/or.17.5.1083
MLA
Yang, S., Zhou, X., Zhu, H., Quan, L., Bai, J., He, J., Gao, Y., Cheng, S., Xu, N."Amplification and overexpression of Aurora-A in esophageal squamous cell carcinoma". Oncology Reports 17.5 (2007): 1083-1088.
Chicago
Yang, S., Zhou, X., Zhu, H., Quan, L., Bai, J., He, J., Gao, Y., Cheng, S., Xu, N."Amplification and overexpression of Aurora-A in esophageal squamous cell carcinoma". Oncology Reports 17, no. 5 (2007): 1083-1088. https://doi.org/10.3892/or.17.5.1083