Anti-proliferative and antioxidant activities of Saposhnikovia divaricata

  • Authors:
    • Joseph Tai
    • Susan Cheung
  • View Affiliations

  • Published online on: July 1, 2007     https://doi.org/10.3892/or.18.1.227
  • Pages: 227-234
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Abstract

The dry root of Saposhnikovia divaricata (Turcz.) Schischk. (SD, syn. Ledebouriella divaricata (Turcz.); Umbelliferae), Siler, a perennial herb of the carrot family, is also known as Fang Feng in traditional Chinese herbal medicine. It is a herbal ingredient included in many polyherb formulae. This study investigated the in vitro anti-proliferative, antioxidant and anti-inflammatory activities of the SD extract (1 g/10 ml 70% ethanol). IC50 (50% inhibition) is estimated at 1/300, 1/1400, 1/250 and 1/600 dilutions, for the K562, HL60, MCF7 and MDA-MB-468 cell lines, respectively. The combination of non-cytotoxic concentrations of SD with chemotherapeutic drugs such as camptothecin or paclitaxel showed additive anti-proliferative effects on K562, HL60 and MCF7 cells, and antagonistic effects on MDA-MB-468 cells. At a dilution of 1/2000, SD induced a differentiation of 17.5±2.5% in HL60 cells along the granulocyte lineage compared to 2.8±0.8% in the untreated controls, but not along the monocyte/macrophage lineage. At non-cytotoxic 1/10000, 1/5000 and 1/2000 dilutions, the SD extract did not affect nitric oxide (NO) production by non-stimulated RAW 264.7 cells, but dose-dependently and significantly reduced NO production by lipopolysaccharide (LPS)-activated RAW 264.7 cells. RT-PCR analyses showed that SD at a dilution of 1/2000 did not affect TNFα, IL-1β, iNOS and COX-2 mRNA expression in RAW 264.7 cells compared to the unstimulated controls, but significantly reduced (p<0.05) iNOS and its mRNA expression in LPS-activated cells. It is concluded that the SD ethanol extract possesses strong anti-proliferative properties against several human tumor cell lines, a mild granulocyte differentiation inducing property on HL60 cells, and potent antioxidant, anti-inflammatory and protective properties on LPS-activated RAW 264.7 cells. Further research is required in order to identify the major ingredients present in the Saposhnikovia divaricata root and rhizome showing the observed activities.

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July 2007
Volume 18 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tai J and Cheung S: Anti-proliferative and antioxidant activities of Saposhnikovia divaricata. Oncol Rep 18: 227-234, 2007.
APA
Tai, J., & Cheung, S. (2007). Anti-proliferative and antioxidant activities of Saposhnikovia divaricata. Oncology Reports, 18, 227-234. https://doi.org/10.3892/or.18.1.227
MLA
Tai, J., Cheung, S."Anti-proliferative and antioxidant activities of Saposhnikovia divaricata". Oncology Reports 18.1 (2007): 227-234.
Chicago
Tai, J., Cheung, S."Anti-proliferative and antioxidant activities of Saposhnikovia divaricata". Oncology Reports 18, no. 1 (2007): 227-234. https://doi.org/10.3892/or.18.1.227