Combined treatment of dendritoma vaccine and low-dose interleukin-2 in stage IV renal cell carcinoma patients induced clinical response: A pilot study

  • Authors:
    • Yanzhang C. Wei
    • Robert P. Sticca
    • Jinhua Li
    • Lillia M. Holmes
    • Kelly E. Burgin
    • Susan Jakubchak
    • Hilary Bouton-Verville
    • Jane Williamson
    • Karen Meyer
    • Lyndon Evans
    • Julie Martin
    • Joseph J. Stephenson
    • Steven Trocha
    • Sam Smith
    • Thomas E. Wagner
  • View Affiliations

  • Published online on: September 1, 2007     https://doi.org/10.3892/or.18.3.665
  • Pages: 665-671
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Abstract

Vaccination using dendritic/tumor cell hybrids represents a novel and promising cancer immunotherapy. We have developed a technology that can instantly purify the hybrids (dendritomas) from the fusion mixture of dendritic cells (DCs) and tumor cells. Our animal studies and a phase I study of stage IV melanoma patients demonstrated that dendritoma vaccination could be conducted without major toxicity and induced tumor cell-specific immunological and clinical responses. In this pilot study, ten stage IV renal cell carcinoma patients were studied. Dendritomas were made from autologous DCs and tumor cells and administered by subcutaneous injection. After initial vaccination, three escalating doses of IL-2 (3, 6, and 9 million units each) were followed within five days. This treatment regimen was tolerated well without severe adverse events directly related to the dendritoma vaccine. Most adverse events were related to IL-2 administration or pre-existing disease. Patient-specific immune responses were evaluated by flow cytometric measurement of interferon-γ-producing T-cells before and after vaccination in response to stimulation with tumor antigens. Nine out of nine patients eligible for the analysis showed an increase of IFN-γ-expressing CD4+ T cells after vaccination(s); while five out of eight patients eligible for the analysis showed an increase of IFN-γ-expressing CD8+ T cells. Clinical responses were documented in 40% of the patients, three with stabilization of disease and one with a partial response documented by a reduction in tumor size. This pilot study demonstrated that dendritoma vaccines could be administered safely to patients with metastatic renal cell carcinoma, while producing both clinical and immunologic evidence of response.

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September 2007
Volume 18 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wei YC, Sticca RP, Li J, Holmes LM, Burgin KE, Jakubchak S, Bouton-Verville H, Williamson J, Meyer K, Evans L, Evans L, et al: Combined treatment of dendritoma vaccine and low-dose interleukin-2 in stage IV renal cell carcinoma patients induced clinical response: A pilot study. Oncol Rep 18: 665-671, 2007
APA
Wei, Y.C., Sticca, R.P., Li, J., Holmes, L.M., Burgin, K.E., Jakubchak, S. ... Wagner, T.E. (2007). Combined treatment of dendritoma vaccine and low-dose interleukin-2 in stage IV renal cell carcinoma patients induced clinical response: A pilot study. Oncology Reports, 18, 665-671. https://doi.org/10.3892/or.18.3.665
MLA
Wei, Y. C., Sticca, R. P., Li, J., Holmes, L. M., Burgin, K. E., Jakubchak, S., Bouton-Verville, H., Williamson, J., Meyer, K., Evans, L., Martin, J., Stephenson, J. J., Trocha, S., Smith, S., Wagner, T. E."Combined treatment of dendritoma vaccine and low-dose interleukin-2 in stage IV renal cell carcinoma patients induced clinical response: A pilot study". Oncology Reports 18.3 (2007): 665-671.
Chicago
Wei, Y. C., Sticca, R. P., Li, J., Holmes, L. M., Burgin, K. E., Jakubchak, S., Bouton-Verville, H., Williamson, J., Meyer, K., Evans, L., Martin, J., Stephenson, J. J., Trocha, S., Smith, S., Wagner, T. E."Combined treatment of dendritoma vaccine and low-dose interleukin-2 in stage IV renal cell carcinoma patients induced clinical response: A pilot study". Oncology Reports 18, no. 3 (2007): 665-671. https://doi.org/10.3892/or.18.3.665