Comparative genomic hybridization, BRAF, RAS, RET, and oligo-array analysis in aneuploid papillary thyroid carcinomas

  • Authors:
    • Raquel Rodrigues
    • Lúcia Roque
    • Carla Espadinha
    • António Pinto
    • Rita Domingues
    • Joana Dinis
    • Ana Catarino
    • Teresa Pereira
    • Valeriano Leite
  • View Affiliations

  • Published online on: October 1, 2007     https://doi.org/10.3892/or.18.4.917
  • Pages: 917-926
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Abstract

Aneuploidy in papillary thyroid carcinomas (PTCs) is considered a marker of worse prognosis. Multiple genetic surveys have been performed in PTCs, however, we are not aware of any such studies in aneuploid PTCs. In order to contribute to a better comprehension of the genetic basis of this neoplasm's more aggressive behaviour in 17 aneuploid PTCs we performed a comparative genomic hybridization (CGH) analysis, studied the BRAF and RAS mutational status, searched for RET/PTC1 and RET/PTC3 rearrangements and determined their expression profile. Array results were validated by TaqMan and immunohistochemistry. CGH revealed multiple non-random chromosomal abnormalities. BRAFV600E and RAS mutations were found in 41.2% and 33% of the carcinomas respectively. None of the studied cases presented RET/PTC1 or RET/PTC3 rearrangement. When comparing array data with the chromosomal, mutational and clinical data we found that: a) loss of control of cellular transcription was of major relevance in this group of neoplasms, HMGA2 being one of the most overexpressed genes; b) gene expression correlated with the mutational status of PTCs, as in BRAF+ cases cMET and FN1 were concomitantly overexpressed; and c) death from disease and distant metastasis was associated to the overexpression of DDR2 and to the down-regulation of genes involved in immune, inflammatory response, signal transduction and cell adhesion processes. In conclusion we have identified in aneuploid PTCs a group of significantly altered molecules that may represent preferential targets for the development of new more efficient therapies in this type of cancer.

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October 2007
Volume 18 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Rodrigues R, Roque L, Espadinha C, Pinto A, Domingues R, Dinis J, Catarino A, Pereira T and Leite V: Comparative genomic hybridization, BRAF, RAS, RET, and oligo-array analysis in aneuploid papillary thyroid carcinomas. Oncol Rep 18: 917-926, 2007
APA
Rodrigues, R., Roque, L., Espadinha, C., Pinto, A., Domingues, R., Dinis, J. ... Leite, V. (2007). Comparative genomic hybridization, BRAF, RAS, RET, and oligo-array analysis in aneuploid papillary thyroid carcinomas. Oncology Reports, 18, 917-926. https://doi.org/10.3892/or.18.4.917
MLA
Rodrigues, R., Roque, L., Espadinha, C., Pinto, A., Domingues, R., Dinis, J., Catarino, A., Pereira, T., Leite, V."Comparative genomic hybridization, BRAF, RAS, RET, and oligo-array analysis in aneuploid papillary thyroid carcinomas". Oncology Reports 18.4 (2007): 917-926.
Chicago
Rodrigues, R., Roque, L., Espadinha, C., Pinto, A., Domingues, R., Dinis, J., Catarino, A., Pereira, T., Leite, V."Comparative genomic hybridization, BRAF, RAS, RET, and oligo-array analysis in aneuploid papillary thyroid carcinomas". Oncology Reports 18, no. 4 (2007): 917-926. https://doi.org/10.3892/or.18.4.917