Multicolor interphase cytogenetics for the study of plasma cell dyscrasias

  • Authors:
    • Borja Sáez
    • José I. Martín-Subero
    • María D. Odero
    • Felipe Prosper
    • Juan C. Cigudosa
    • Robert Schoch
    • María J. Calasanz
    • Reiner Siebert
  • View Affiliations

  • Published online on: November 1, 2007     https://doi.org/10.3892/or.18.5.1099
  • Pages: 1099-1106
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Abstract

Specific chromosomal abnormalities such as chromosome 13 deletions and some translocations affecting the immunoglobulin heavy chain (IGH) gene, namely t(4;14)(p16;q32) and t(14;16)(q32;q23) have been associated with an adverse prognosis in multiple myeloma. Conventional cytogenetic techniques fail to detect these aberrations in the majority of cases. Thus, we have developed a novel set of interphase fluorescence in situ hybridization (I-FISH) assays targeting those regions frequently lost on chromosome 13 as well as those oncogenes most recurrently involved in translocations with the IGH locus in multiple myeloma, i.e. IRTA1/2 (1q21), FGFR3/MMSET (4p16), CCND3 (6p21), IRF4 (6p25), CCND1 (11q13), MAF (16q23), and MAFB (20q12). The probes were combined in a multicolor fashion to develop novel multicolor I-FISH (MI-FISH) assays, whose validity and applicability was evaluated in negative controls and in a series of 13 plasma cell neoplasias. Additionally, a combination of the novel MI-FISH assays with staining for the plasma cell-specific antigen VS38c by means of multicolor FICTION (M-FICTION, fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasms) allowed us to selectively analyze the plasma cell compartment, and thereby to increase the assay sensitivity.

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November 2007
Volume 18 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Sáez B, Martín-Subero JI, Odero MD, Prosper F, Cigudosa JC, Schoch R, Calasanz MJ and Siebert R: Multicolor interphase cytogenetics for the study of plasma cell dyscrasias. Oncol Rep 18: 1099-1106, 2007
APA
Sáez, B., Martín-Subero, J.I., Odero, M.D., Prosper, F., Cigudosa, J.C., Schoch, R. ... Siebert, R. (2007). Multicolor interphase cytogenetics for the study of plasma cell dyscrasias. Oncology Reports, 18, 1099-1106. https://doi.org/10.3892/or.18.5.1099
MLA
Sáez, B., Martín-Subero, J. I., Odero, M. D., Prosper, F., Cigudosa, J. C., Schoch, R., Calasanz, M. J., Siebert, R."Multicolor interphase cytogenetics for the study of plasma cell dyscrasias". Oncology Reports 18.5 (2007): 1099-1106.
Chicago
Sáez, B., Martín-Subero, J. I., Odero, M. D., Prosper, F., Cigudosa, J. C., Schoch, R., Calasanz, M. J., Siebert, R."Multicolor interphase cytogenetics for the study of plasma cell dyscrasias". Oncology Reports 18, no. 5 (2007): 1099-1106. https://doi.org/10.3892/or.18.5.1099