17-β-estradiol affects nuclear image properties in MCF-10F human breast epithelial cells with tumorigenesis

  • Authors:
    • Maria Luiza S. Mello
    • Patricia Russo
    • Jose Russo
    • Benedicto C. Vidal
  • View Affiliations

  • Published online on: December 1, 2007     https://doi.org/10.3892/or.18.6.1475
  • Pages: 1475-1481
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Abstract

Treatment of the human breast epithelial cells MCF-10F with 17-β-estradiol (E2) induces transformation and tumorigenesis. E2-transformed MCF-10F cells are known to exhibit progressive loss of ductulogenesis, and invasive and tumorigenic phenotypes. Although DNA amounts and chromatin supraorganization change in E2-transformed MCF cells, no comparative study has yet been undertaken in the resulting cells selected for aggressive invasiveness (C5) and tumor generation in a heterologous host. The aim of this study was thus to determine whether changes in Feulgen-DNA content and chromatin supraorganization are involved during E2-induced transformation and tumorigenesis of the MCF-10F cells. Image analysis was performed for nontransformed and E2-transformed MCF cells, highly invasive cells (C5), and for cell lines (C5-A6-T6 and C5-A8-T8) derived from tumors generated by injection of C5 cells in SCID mice. A decrease in Feulgen-DNA amounts and nuclear sizes induced by E2 treatment was accented with selection of the highly invasive tumorigenesis potential. However, in the tumor-derived cells a high variability in cellular phenotypes resulted inclusively in near-polyploidy. Significant changes in textural parameters, including nuclear entropy, indicated chromatin structural remodeling with advancing tumorigenesis. An increased variability in the degree of chromatin packing states in the E2-transformed MCF cells is followed by reduction in chromatin condensation and in contrast between condensed and noncondensed chromatin in the highly invasive C5 cells and tumor-derived cell lines. Studies on epigenetic mechanisms involving DNA methylation and/or the histone code would contribute to a better interpretation of the chromatin supraorganization changes reported herein.

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December 2007
Volume 18 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Mello ML, Russo P, Russo J and Vidal BC: 17-β-estradiol affects nuclear image properties in MCF-10F human breast epithelial cells with tumorigenesis. Oncol Rep 18: 1475-1481, 2007
APA
Mello, M.L., Russo, P., Russo, J., & Vidal, B.C. (2007). 17-β-estradiol affects nuclear image properties in MCF-10F human breast epithelial cells with tumorigenesis. Oncology Reports, 18, 1475-1481. https://doi.org/10.3892/or.18.6.1475
MLA
Mello, M. L., Russo, P., Russo, J., Vidal, B. C."17-β-estradiol affects nuclear image properties in MCF-10F human breast epithelial cells with tumorigenesis". Oncology Reports 18.6 (2007): 1475-1481.
Chicago
Mello, M. L., Russo, P., Russo, J., Vidal, B. C."17-β-estradiol affects nuclear image properties in MCF-10F human breast epithelial cells with tumorigenesis". Oncology Reports 18, no. 6 (2007): 1475-1481. https://doi.org/10.3892/or.18.6.1475