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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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December 2007 Volume 18 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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December 2007 Volume 18 Issue 6

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Article

Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells

  • Authors:
    • Michael W.Y. Chan
    • Christine Y.P. Wong
    • Alfred S.L. Cheng
    • Victor Y.W. Chan
    • Ka K. Chan
    • Ka F. To
    • Francis K.L. Chan
    • Joseph J.Y. Sung
    • Wai K. Leung

  • View Affiliations / Copyright

    Affiliations: Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, P.R. China
  • Pages: 1557-1562
    |
    Published online on: December 1, 2007
       https://doi.org/10.3892/or.18.6.1557
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Abstract

Although selective cyclooxygenase-2 (COX-2) inhibitors suppress cell proliferation in gastric cancer, it remains debatable whether their effect is mediated through COX-2 dependent or independent pathways. We investigated the effects of the targeted inhibition of COX-2 expression by small interfering RNA (siRNA) in human gastric cancer cells and compared it to the effects of treatment with a specific COX-2 inhibitor. COX-2 mRNA and proteins were significantly reduced by up to 80% on day 2 after COX-2 siRNA transfection to the gastric cancer cell line MKN45. Concentrations of prostaglandins E2 (PGE2) in the condition medium were also reduced to 30% after siRNA transfection. Transfection of COX-2 siRNA exhibited a more potent anti-proliferative effect on MKN45 cells than treatment with high-dose (100 µM) NS398. COX-2 siRNA also significantly reduced tumor growth in nude mice. While COX-2 siRNA transfection alone had no obvious pro-apoptotic effects, unlike low-dose (10 µM) NS398 it enhanced the apoptotic reaction of MKN45 cells to cisplatin therapy. In conclusion, our results demonstrate for the first time that COX-2 siRNA inhibits cell growth and enhances the chemosensitivity of gastric cancer cells. RNA interference may be a promising alternative to specific COX-2 inhibitors in the prevention and treatment of gastric cancer.

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Copy and paste a formatted citation
Spandidos Publications style
Chan MW, Wong CY, Cheng AS, Chan VY, Chan KK, To KF, Chan FK, Sung JJ and Leung WK: Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells. Oncol Rep 18: 1557-1562, 2007.
APA
Chan, M.W., Wong, C.Y., Cheng, A.S., Chan, V.Y., Chan, K.K., To, K.F. ... Leung, W.K. (2007). Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells. Oncology Reports, 18, 1557-1562. https://doi.org/10.3892/or.18.6.1557
MLA
Chan, M. W., Wong, C. Y., Cheng, A. S., Chan, V. Y., Chan, K. K., To, K. F., Chan, F. K., Sung, J. J., Leung, W. K."Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells". Oncology Reports 18.6 (2007): 1557-1562.
Chicago
Chan, M. W., Wong, C. Y., Cheng, A. S., Chan, V. Y., Chan, K. K., To, K. F., Chan, F. K., Sung, J. J., Leung, W. K."Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells". Oncology Reports 18, no. 6 (2007): 1557-1562. https://doi.org/10.3892/or.18.6.1557
Copy and paste a formatted citation
x
Spandidos Publications style
Chan MW, Wong CY, Cheng AS, Chan VY, Chan KK, To KF, Chan FK, Sung JJ and Leung WK: Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells. Oncol Rep 18: 1557-1562, 2007.
APA
Chan, M.W., Wong, C.Y., Cheng, A.S., Chan, V.Y., Chan, K.K., To, K.F. ... Leung, W.K. (2007). Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells. Oncology Reports, 18, 1557-1562. https://doi.org/10.3892/or.18.6.1557
MLA
Chan, M. W., Wong, C. Y., Cheng, A. S., Chan, V. Y., Chan, K. K., To, K. F., Chan, F. K., Sung, J. J., Leung, W. K."Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells". Oncology Reports 18.6 (2007): 1557-1562.
Chicago
Chan, M. W., Wong, C. Y., Cheng, A. S., Chan, V. Y., Chan, K. K., To, K. F., Chan, F. K., Sung, J. J., Leung, W. K."Targeted inhibition of COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells". Oncology Reports 18, no. 6 (2007): 1557-1562. https://doi.org/10.3892/or.18.6.1557
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