Hyperthermic CO2 pneumoperitoneum induces apoptosis in human colon cancer cells through Bax-associated mitochondrial pathway

  • Authors:
    • Yuanfei Peng
    • Minhua Zheng
    • Bo Feng
    • Xuehua Chen
    • Beiqin Yu
    • Aiguo Lu
    • Minliang Wang
    • Jianwen Li
    • Junjun Ma
    • Lie Xu
  • View Affiliations

  • Published online on: January 1, 2008     https://doi.org/10.3892/or.19.1.73
  • Pages: 73-79
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Abstract

Peritoneal carcinomatosis of colorectal cancer is common and associated with poor prognosis, which poses a serious challenge and satisfactory treatments are urgently needed. Hyperthermic CO2 pneumoperitoneum (HT-CO2) is a new strategy. This study was designed to determine the potential of HT-CO2 against colorectal cancer cells. Based on an in vitro HT-CO2 study model, the anti-tumor efficacy of HT-CO2 (42-44°C for 2-4 h) on human colon cancer COLO 205 cells was evaluated and the mechanisms of actions were analyzed. We found that HT-CO2 (43-44°C for 2-4 h) significantly decreased cell viability as determined by WST-8 assay, and the cytotoxicity was attributable to HT-CO2-induced hyperthermia and extracellular acidification. Apoptosis was the major form of cell killing as demonstrated by Annexin-V/PI flow cytometry and morphological analysis (Hoechst/PI fluorescence microscopy and transmission electron microscopy). Further Western blot analysis and flow cytometric analysis of mitochondrial membrane potential showed that Bax-associated mitochondrial apoptotic pathway played critical role in the induction of apoptosis. We conclude that HT-CO2 has significant cytotoxic effect on colon cancer cells through induction of Bax-associated mitochondrial apoptosis, and the cytocidal effect is attributable to HT-CO2-induced hyperthermia and extracellular acidifications. Our data suggest that HT-CO2 may serve as a potential candidate for treating and/or preventing peritoneal carcinomatosis of colorectal cancer and further investigations both in vitro as well as in vivo in animal models are needed.

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January 2008
Volume 19 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Peng Y, Zheng M, Feng B, Chen X, Yu B, Lu A, Wang M, Li J, Ma J, Xu L, Xu L, et al: Hyperthermic CO2 pneumoperitoneum induces apoptosis in human colon cancer cells through Bax-associated mitochondrial pathway. Oncol Rep 19: 73-79, 2008
APA
Peng, Y., Zheng, M., Feng, B., Chen, X., Yu, B., Lu, A. ... Xu, L. (2008). Hyperthermic CO2 pneumoperitoneum induces apoptosis in human colon cancer cells through Bax-associated mitochondrial pathway. Oncology Reports, 19, 73-79. https://doi.org/10.3892/or.19.1.73
MLA
Peng, Y., Zheng, M., Feng, B., Chen, X., Yu, B., Lu, A., Wang, M., Li, J., Ma, J., Xu, L."Hyperthermic CO2 pneumoperitoneum induces apoptosis in human colon cancer cells through Bax-associated mitochondrial pathway". Oncology Reports 19.1 (2008): 73-79.
Chicago
Peng, Y., Zheng, M., Feng, B., Chen, X., Yu, B., Lu, A., Wang, M., Li, J., Ma, J., Xu, L."Hyperthermic CO2 pneumoperitoneum induces apoptosis in human colon cancer cells through Bax-associated mitochondrial pathway". Oncology Reports 19, no. 1 (2008): 73-79. https://doi.org/10.3892/or.19.1.73