Anti-tumor effects of cimetidine on hepatocellular carcinomas in diethylnitrosamine-treated rats

  • Authors:
    • Koichiro Furuta
    • Shuichi Sato
    • Tatsuya Miyake
    • Eisuke Okamoto
    • Junichi Ishine
    • Shunji Ishihara
    • Yuji Amano
    • Kyoichi Adachi
    • Yoshikazu Kinoshita
  • View Affiliations

  • Published online on: February 1, 2008     https://doi.org/10.3892/or.19.2.361
  • Pages:361-368
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Abstract

Cimetidine is known to have an anti-tumor effect on certain types of malignancies, though on hepatocellular carcinomas (HCCs), its effect remains unclear. We studied the anti-tumor effects of cimetidine on chemically-induced HCCs in rats. Four-week-old male Wistar rats (n=105) were divided into 4 groups. Those in groups A and B were administered diethylnitrosamine (DEN) intraperitoneally at 100 mg/kg body weight every week for 6 weeks, during which rats in group A were given tap water and those in group B received cimetidine (100 mg/kg/day) in their drinking water. Rats in groups C and D were administered saline instead of DEN and given tap water with 100 mg/kg/day of cimetidine, respectively. The animals were sacrificed at 7, 12, 22 and 32 weeks after the first administration of drugs and examined. Liver nodules were observed only in groups A and B, with the number of nodules, maximum diameter of the largest nodule, and liver weight significantly lower in group B. Immunohistochemistry findings showed that glutathione S-transferase placental-positive preneoplastic foci were significantly decreased in group B. Cimetidine treatment decreased the number of proliferating cell nuclear antigen-positive hepatocytes and tended to enhance natural killer (NK) cell activity in splenic lymphocytes. In addition, flow cytometry revealed that the proportion of NK cells among total splenic lympocytes was not affected by cimetidine treatment. Our results showed that cimetidine has an inhibiting effect on hepatocarcinogenesis.

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February 2008
Volume 19 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Furuta, K., Sato, S., Miyake, T., Okamoto, E., Ishine, J., Ishihara, S. ... Kinoshita, Y. (2008). Anti-tumor effects of cimetidine on hepatocellular carcinomas in diethylnitrosamine-treated rats. Oncology Reports, 19, 361-368. https://doi.org/10.3892/or.19.2.361
MLA
Furuta, K., Sato, S., Miyake, T., Okamoto, E., Ishine, J., Ishihara, S., Amano, Y., Adachi, K., Kinoshita, Y."Anti-tumor effects of cimetidine on hepatocellular carcinomas in diethylnitrosamine-treated rats". Oncology Reports 19.2 (2008): 361-368.
Chicago
Furuta, K., Sato, S., Miyake, T., Okamoto, E., Ishine, J., Ishihara, S., Amano, Y., Adachi, K., Kinoshita, Y."Anti-tumor effects of cimetidine on hepatocellular carcinomas in diethylnitrosamine-treated rats". Oncology Reports 19, no. 2 (2008): 361-368. https://doi.org/10.3892/or.19.2.361