ESTABLISHMENT OF HUMAN BLADDER-CANCER CELL-LINE (HAMT-1) AND LONG-TERM SUBCULTURE IN NUDE-MICE
- H ITO
- T KOTAKE
- M MASAI
- T MIZUTA
- M ADACHI
Affiliations: JAPAN IMMUNORES LABS CO LTD,TAKASAKI,JAPAN.
- Published online on: March 1, 1995 https://doi.org/10.3892/or.2.2.221
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Human bladder cancer cell line (HAMT-1) was established from a transitional cell carcinoma of the bladder (T4N3M0, grade 3) of a 61-year-old male by using a serum-free medium. HAMT-1 cells in serum-free medium were non-adhesive to the flask wall or to other cells. The cells grew in attached form by the addition of fetal calf serum. Heterotransplantation of HAMT-1 cells into nude mice and the long-term subculture were successful. Tumor bearing nude mice showed an elevated serum tissue polypeptide antigen as in the patient.