ESTABLISHMENT OF HUMAN BLADDER-CANCER CELL-LINE (HAMT-1) AND LONG-TERM SUBCULTURE IN NUDE-MICE

  • Authors:
    • H ITO
    • T KOTAKE
    • M MASAI
    • T MIZUTA
    • M ADACHI
  • View Affiliations

  • Published online on: March 1, 1995     https://doi.org/10.3892/or.2.2.221
  • Pages: 221-226
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Abstract

Human bladder cancer cell line (HAMT-1) was established from a transitional cell carcinoma of the bladder (T4N3M0, grade 3) of a 61-year-old male by using a serum-free medium. HAMT-1 cells in serum-free medium were non-adhesive to the flask wall or to other cells. The cells grew in attached form by the addition of fetal calf serum. Heterotransplantation of HAMT-1 cells into nude mice and the long-term subculture were successful. Tumor bearing nude mice showed an elevated serum tissue polypeptide antigen as in the patient.

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March 1995
Volume 2 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
ITO H, KOTAKE T, MASAI M, MIZUTA T and ADACHI M: ESTABLISHMENT OF HUMAN BLADDER-CANCER CELL-LINE (HAMT-1) AND LONG-TERM SUBCULTURE IN NUDE-MICE. Oncol Rep 2: 221-226, 1995
APA
ITO, H., KOTAKE, T., MASAI, M., MIZUTA, T., & ADACHI, M. (1995). ESTABLISHMENT OF HUMAN BLADDER-CANCER CELL-LINE (HAMT-1) AND LONG-TERM SUBCULTURE IN NUDE-MICE. Oncology Reports, 2, 221-226. https://doi.org/10.3892/or.2.2.221
MLA
ITO, H., KOTAKE, T., MASAI, M., MIZUTA, T., ADACHI, M."ESTABLISHMENT OF HUMAN BLADDER-CANCER CELL-LINE (HAMT-1) AND LONG-TERM SUBCULTURE IN NUDE-MICE". Oncology Reports 2.2 (1995): 221-226.
Chicago
ITO, H., KOTAKE, T., MASAI, M., MIZUTA, T., ADACHI, M."ESTABLISHMENT OF HUMAN BLADDER-CANCER CELL-LINE (HAMT-1) AND LONG-TERM SUBCULTURE IN NUDE-MICE". Oncology Reports 2, no. 2 (1995): 221-226. https://doi.org/10.3892/or.2.2.221