RELATIONSHIP BETWEEN MDR-1 MESSENGER-RNA EXPRESSION AND ANTICANCER DRUG-SENSITIVITY IN HUMAN HEPATOCELLULAR-CARCINOMA CELL-LINES

  • Authors:
    • F SASAKI
    • H ISHIMURA
    • Y HATA
    • J UCHINO
  • View Affiliations

  • Published online on: March 1, 1995     https://doi.org/10.3892/or.2.2.309
  • Pages: 309-312
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We examined the relationship between chemosensitivity and the expression of mdr-1 and P-gp in ten human HCC cell lines. Expression of P-gp was detected immunohistochemically and the expression of mdr-1 mRNA by the reverse transcriptase polymerase chain reaction (RT-PCR). Chemosensitivity was determined by the MTT assay. P-gp was positive in nine of the ten lines. mdr-1 mRNA expression was found in five. All cells lines positive for mdr-1 mRNA expression expressed P-gp. We could not demonstrate a correlation between mdr-1 or P-gp expression and drug resistance. We conclude that drug-resistance in HCC cells depends not only on mdr-1 but also on other factor(s).

Related Articles

Journal Cover

March 1995
Volume 2 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
SASAKI F, ISHIMURA H, HATA Y and UCHINO J: RELATIONSHIP BETWEEN MDR-1 MESSENGER-RNA EXPRESSION AND ANTICANCER DRUG-SENSITIVITY IN HUMAN HEPATOCELLULAR-CARCINOMA CELL-LINES. Oncol Rep 2: 309-312, 1995
APA
SASAKI, F., ISHIMURA, H., HATA, Y., & UCHINO, J. (1995). RELATIONSHIP BETWEEN MDR-1 MESSENGER-RNA EXPRESSION AND ANTICANCER DRUG-SENSITIVITY IN HUMAN HEPATOCELLULAR-CARCINOMA CELL-LINES. Oncology Reports, 2, 309-312. https://doi.org/10.3892/or.2.2.309
MLA
SASAKI, F., ISHIMURA, H., HATA, Y., UCHINO, J."RELATIONSHIP BETWEEN MDR-1 MESSENGER-RNA EXPRESSION AND ANTICANCER DRUG-SENSITIVITY IN HUMAN HEPATOCELLULAR-CARCINOMA CELL-LINES". Oncology Reports 2.2 (1995): 309-312.
Chicago
SASAKI, F., ISHIMURA, H., HATA, Y., UCHINO, J."RELATIONSHIP BETWEEN MDR-1 MESSENGER-RNA EXPRESSION AND ANTICANCER DRUG-SENSITIVITY IN HUMAN HEPATOCELLULAR-CARCINOMA CELL-LINES". Oncology Reports 2, no. 2 (1995): 309-312. https://doi.org/10.3892/or.2.2.309