GROWTH-INHIBITION OF HUMAN-MELANOMA CELLS BY VITAMIN-D ANALOGS

  • Authors:
    • RV BRENNER
    • M SHABAHANG
    • AM HOUGHTON
    • RJ NAUTA
    • MR USKOKOVIC
    • LM SCHUMAKER
    • RR BURAS
    • SRT EVANS
  • View Affiliations

  • Published online on: November 1, 1995     https://doi.org/10.3892/or.2.6.1157
  • Pages: 1157-1162
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Abstract

The antiproliferative activity of 1,25(OH)(2)-vitamin D-3, and four vitamin D analogs was assessed in RPMI-7951, a human melanoma cell line which expresses the vitamin D receptor. Proliferation assays consisted of a [H-3]-thymidine incorporation assay, and a 6-day growth study. The affinity of vitamin D analogs for vitamin D receptor relative to 125(OH)(2)-vitamin D-3 was determined with a hydroxyapatite-based competitive binding assay. For the proliferation assays, cells were treated with 10(-8) M 1,25(OH)(2)-vitamin D-3, 1,25(OH)(2)-16-ene-23-yne-vitamin D-3 (Ro 23-7553), 1,25(OH)(2)-16-ene-23-yne-26,27-hexafluoro-vitamin D-3 (Ro 24-5531), 1,25(OH),-16,23Z-diene-26,27-hexafluoro-vitamin D-3 (Ro 25-5317), and 1 alpha-fluoro-25(OH)- 16-ene-23-yne-hexafluoro-vitamin D-3 (Ro 24-5583). 1,25(OH)(2)-vitamin D-3 and the four analogs all significantly inhibited melanoma cell growth (P<0.05). Competitive binding of the vitamin D analogs to vitamin D receptor ranged from 51% to 72% that of 1,25(OH)(2)-vitamin D-3, suggesting a receptor-mediated response. These results demonstrate that analogs of 1,25(OH)(2)-vitamin D-3 are potent antiproliferative agents in human melanoma cells in vitro.

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November 1995
Volume 2 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
BRENNER R, SHABAHANG M, HOUGHTON A, NAUTA R, USKOKOVIC M, SCHUMAKER L, BURAS R and EVANS S: GROWTH-INHIBITION OF HUMAN-MELANOMA CELLS BY VITAMIN-D ANALOGS. Oncol Rep 2: 1157-1162, 1995
APA
BRENNER, R., SHABAHANG, M., HOUGHTON, A., NAUTA, R., USKOKOVIC, M., SCHUMAKER, L. ... EVANS, S. (1995). GROWTH-INHIBITION OF HUMAN-MELANOMA CELLS BY VITAMIN-D ANALOGS. Oncology Reports, 2, 1157-1162. https://doi.org/10.3892/or.2.6.1157
MLA
BRENNER, R., SHABAHANG, M., HOUGHTON, A., NAUTA, R., USKOKOVIC, M., SCHUMAKER, L., BURAS, R., EVANS, S."GROWTH-INHIBITION OF HUMAN-MELANOMA CELLS BY VITAMIN-D ANALOGS". Oncology Reports 2.6 (1995): 1157-1162.
Chicago
BRENNER, R., SHABAHANG, M., HOUGHTON, A., NAUTA, R., USKOKOVIC, M., SCHUMAKER, L., BURAS, R., EVANS, S."GROWTH-INHIBITION OF HUMAN-MELANOMA CELLS BY VITAMIN-D ANALOGS". Oncology Reports 2, no. 6 (1995): 1157-1162. https://doi.org/10.3892/or.2.6.1157