Lentivirus-mediated short hairpin RNA targeting the APRIL gene suppresses the growth of pancreatic cancer cells in vitro and in vivo

  • Authors:
    • Feng Wang
    • Lin Chen
    • Zhen-Biao Mao
    • Jian-Guo Shao
    • Chang Tan
    • Wei-Da Huang
  • View Affiliations

  • Published online on: July 1, 2008     https://doi.org/10.3892/or.20.1.135
  • Pages: 135-139
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Abstract

RNA interference (RNAi) is an evolutionarily conserved process of gene silencing in multiple organisms, which has become a powerful tool for investigating gene function by reverse genetics. Herein, we constructed a short hairpin RNA (shRNA) lentiviral expression vector targeting a proliferation-inducing ligand (APRIL) gene in the CFPAC-1 cell (a type of cell strain of human pancreatic cancer) in order to observe the inhibitory effect of APRIL gene's shRNA on the growth of the CFPAC-1 cell in vitro and in vivo. The results showed that lentivirus-mediated RNAi effectively inhibited the expression of APRIL mRNA and protein in CFPAC-1 cells. Moreover, it can inhibit the growth of pancreatic cancer cells in vitro and in vivo. Our study indicates that lentivirus-mediated gene therapy is an attractive strategy in the treatment of pancreatic cancer and justifies the use of lentivirus in cancer gene therapy studies.

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July 2008
Volume 20 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang F, Chen L, Mao Z, Shao J, Tan C and Huang W: Lentivirus-mediated short hairpin RNA targeting the APRIL gene suppresses the growth of pancreatic cancer cells in vitro and in vivo. Oncol Rep 20: 135-139, 2008
APA
Wang, F., Chen, L., Mao, Z., Shao, J., Tan, C., & Huang, W. (2008). Lentivirus-mediated short hairpin RNA targeting the APRIL gene suppresses the growth of pancreatic cancer cells in vitro and in vivo. Oncology Reports, 20, 135-139. https://doi.org/10.3892/or.20.1.135
MLA
Wang, F., Chen, L., Mao, Z., Shao, J., Tan, C., Huang, W."Lentivirus-mediated short hairpin RNA targeting the APRIL gene suppresses the growth of pancreatic cancer cells in vitro and in vivo". Oncology Reports 20.1 (2008): 135-139.
Chicago
Wang, F., Chen, L., Mao, Z., Shao, J., Tan, C., Huang, W."Lentivirus-mediated short hairpin RNA targeting the APRIL gene suppresses the growth of pancreatic cancer cells in vitro and in vivo". Oncology Reports 20, no. 1 (2008): 135-139. https://doi.org/10.3892/or.20.1.135