Effective enhancement of X-ray-induced apoptosis in human cancer cells with mutated p53 by siRNA targeting XIAP

  • Authors:
    • Ken Ohnishi
    • Yoko Nagata
    • Akihisa Takahashi
    • Shigeki Taniguchi
    • Takeo Ohnishi
  • View Affiliations

  • Published online on: July 1, 2008     https://doi.org/10.3892/or.20.1.57
  • Pages: 57-61
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The X-chromosome-linked inhibitor of the apoptosis protein (XIAP) is known to be an inhibitory factor for caspase-3. The aim of our study was to see whether radiation-induced apoptosis is enhanced by RNA interference targeting the XIAP through an elevation of caspase-3 activity, and whether the effect of XIAP depression depends on the p53 status of cancer cells. Two types of transformed human cultured non-small cell lung cancer cells (H1299) were used: wild-type p53-transfected cells (H1299/wtp53) and mutated p53-transfected cells (H1299/mp53). When 21-mer siRNA targeting XIAP (XIAP-siRNA) was transfected into these cells using liposomes, a suppression of the constitutive XIAP protein expression was observed. XIAP-siRNA enhanced radiation sensitivity in H1299/wtp53 and in H1299/mp53 cells and was very effective in H1299/mp53 cells. Radiation-induced apoptosis and the activation of caspase-3 were more elevated by XIAP-siRNA in the H1299/mp53 cells than in H1299/ wtp53. These results suggest that XIAP-siRNA is a possible candidate for a radiation sensitizer in cancer radiotherapy, especially in cells with mutated p53.

Related Articles

Journal Cover

July 2008
Volume 20 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ohnishi K, Nagata Y, Takahashi A, Taniguchi S and Ohnishi T: Effective enhancement of X-ray-induced apoptosis in human cancer cells with mutated p53 by siRNA targeting XIAP. Oncol Rep 20: 57-61, 2008
APA
Ohnishi, K., Nagata, Y., Takahashi, A., Taniguchi, S., & Ohnishi, T. (2008). Effective enhancement of X-ray-induced apoptosis in human cancer cells with mutated p53 by siRNA targeting XIAP. Oncology Reports, 20, 57-61. https://doi.org/10.3892/or.20.1.57
MLA
Ohnishi, K., Nagata, Y., Takahashi, A., Taniguchi, S., Ohnishi, T."Effective enhancement of X-ray-induced apoptosis in human cancer cells with mutated p53 by siRNA targeting XIAP". Oncology Reports 20.1 (2008): 57-61.
Chicago
Ohnishi, K., Nagata, Y., Takahashi, A., Taniguchi, S., Ohnishi, T."Effective enhancement of X-ray-induced apoptosis in human cancer cells with mutated p53 by siRNA targeting XIAP". Oncology Reports 20, no. 1 (2008): 57-61. https://doi.org/10.3892/or.20.1.57