Human hair follicle and interfollicular keratinocyte reactivity to mouse HPV16-transformed cells: An in vitro study

  • Authors:
    • Karel Smetana
    • Barbora Dvoránková
    • Lukás Lacina
    • Zdenek Cada
    • Vladimír Vonka
  • View Affiliations

  • Published online on: July 1, 2008     https://doi.org/10.3892/or.20.1.75
  • Pages: 75-80
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Abstract

The role of stem cells in cancer formation and spreading has been established. As with normal tissue, the cancer stem cells need a special microenvironment to support their growth. This microenvironment may be represented by the tumor stroma. One of the possible ways of tumor stromal formation is the epithelial-mesenchymal transition of tumor epithelium. Following this mechanism, stromal cells must share the basic genetic alterations with the tumor cells. In an attempt to create a system capable of testing some aspects of the mesenchymal cell-keratinocyte interactions, we studied the effects of the fibroblastoid mouse TC-1 cells that were prepared by the introduction of human papillomavirus type 16 (HPV16) genes E6 and E7 to lung epithelial cells on the phenotype of normal human interfollicular and hair follicle keratinocytes. From this point of view, they may resemble stromal cells formed by the epithelial-mesenchymal transition of cells from HPV-induced squamous cell carcinoma. In contrast to 3T3 murine embryonic fibroblasts which were used as control cells, TC-1 cells influenced not only the size of the keratinocytes and the shape of their colonies, but also induced the expression of keratins 8 and 19 and vimentin. In conclusion, TC-1 cells exhibited a marked biological activity by influencing the behavior of the normal human follicular and intefollicular keratinocytes. This observation is compatible with the hypothesis that stromal cells play an important role in tumor progression and spreading.

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July 2008
Volume 20 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Smetana K, Dvoránková B, Lacina L, Cada Z and Vonka V: Human hair follicle and interfollicular keratinocyte reactivity to mouse HPV16-transformed cells: An in vitro study. Oncol Rep 20: 75-80, 2008
APA
Smetana, K., Dvoránková, B., Lacina, L., Cada, Z., & Vonka, V. (2008). Human hair follicle and interfollicular keratinocyte reactivity to mouse HPV16-transformed cells: An in vitro study. Oncology Reports, 20, 75-80. https://doi.org/10.3892/or.20.1.75
MLA
Smetana, K., Dvoránková, B., Lacina, L., Cada, Z., Vonka, V."Human hair follicle and interfollicular keratinocyte reactivity to mouse HPV16-transformed cells: An in vitro study". Oncology Reports 20.1 (2008): 75-80.
Chicago
Smetana, K., Dvoránková, B., Lacina, L., Cada, Z., Vonka, V."Human hair follicle and interfollicular keratinocyte reactivity to mouse HPV16-transformed cells: An in vitro study". Oncology Reports 20, no. 1 (2008): 75-80. https://doi.org/10.3892/or.20.1.75