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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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March 2011 Volume 25 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells

  • Authors:
    • Toshikazu Suzuki
    • Jun Lu
    • Gaofeng Hu
    • Kazuko Kita
    • Nobuo Suzuki

  • View Affiliations / Copyright

    Affiliations: Department of Health and Nutrition, Wayo Women's University, 2-3-1 Konodai, Ichikawa, Chiba 272-8533, Japan, Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan
  • Pages: 879-885
    |
    Published online on: January 10, 2011
       https://doi.org/10.3892/or.2011.1134
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Abstract

Glucose-regulated protein 78 (GRP78) is expressed abundantly in various types of cancer cells and is believed to contribute to chemotherapeutic resistance. In this study, we investigated the effect of a continuous approach for the expression of a short hairpin RNA (shRNA) targeted to GRP78 with retrovirus transduction on the sensitivity to the anticancer drugs VP-16 and cisplatin. The reduction of GRP78 expression failed, and the expression of GRP94 and P5 chaperon mRNA increased; this increase was associated with a mild activation of the unfolded protein response in HeLa cells, which were stably transduced with GRP78 shRNA gene. The transduced cells exhibited similar sensitivity to VP-16-induced cell death when compared to control GFP shRNA gene-transduced cells. However, sensitivity to cisplatin-induced cell death was higher in GRP78 shRNA gene-transduced cells compared to control cells. These results demonstrate that the continuous or prolonged approach targeting GRP78 confers sensitization of HeLa cells to cisplatin independently of the down-regulation of GRP78 expression. The role of the unfolded protein response in sensitization to cisplatin is discussed.

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Copy and paste a formatted citation
Spandidos Publications style
Suzuki T, Lu J, Hu G, Kita K and Suzuki N: Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells. Oncol Rep 25: 879-885, 2011.
APA
Suzuki, T., Lu, J., Hu, G., Kita, K., & Suzuki, N. (2011). Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells. Oncology Reports, 25, 879-885. https://doi.org/10.3892/or.2011.1134
MLA
Suzuki, T., Lu, J., Hu, G., Kita, K., Suzuki, N."Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells". Oncology Reports 25.3 (2011): 879-885.
Chicago
Suzuki, T., Lu, J., Hu, G., Kita, K., Suzuki, N."Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells". Oncology Reports 25, no. 3 (2011): 879-885. https://doi.org/10.3892/or.2011.1134
Copy and paste a formatted citation
x
Spandidos Publications style
Suzuki T, Lu J, Hu G, Kita K and Suzuki N: Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells. Oncol Rep 25: 879-885, 2011.
APA
Suzuki, T., Lu, J., Hu, G., Kita, K., & Suzuki, N. (2011). Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells. Oncology Reports, 25, 879-885. https://doi.org/10.3892/or.2011.1134
MLA
Suzuki, T., Lu, J., Hu, G., Kita, K., Suzuki, N."Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells". Oncology Reports 25.3 (2011): 879-885.
Chicago
Suzuki, T., Lu, J., Hu, G., Kita, K., Suzuki, N."Retrovirus-mediated transduction of a short hairpin RNA gene for GRP78 fails to downregulate GRP78 expression but leads to cisplatin sensitization in HeLa cells". Oncology Reports 25, no. 3 (2011): 879-885. https://doi.org/10.3892/or.2011.1134
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