Optimization of hyperthermia and dendritic cell immunotherapy for squamous cell carcinoma

  • Authors:
    • Koushi Matsumoto
    • Noriyuki Yamamoto
    • Sumitaka Hagiwara
    • Masaki Saito
    • Hiroki Furue
    • Toshio Shigetomi
    • Yuji Narita
    • Kenji Mitsudo
    • Iwai Tohnai
    • Takeshi Kobayashi
    • Minoru Ueda
  • View Affiliations

  • Published online on: March 23, 2011     https://doi.org/10.3892/or.2011.1232
  • Pages: 1525-1532
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Abstract

The aims of this study were to explore the feasibility of a novel combination therapy comprising hyperthermia (HT) and dendritic cell (DC) application for squamous cell carcinoma (SCC), and to optimize the conditions of this therapy. In vitro, the correlation between maturation of DCs by co-culture with SCCVII cells and HT was investigated. DCs did not mature in simple HT (43˚C for 30 min) with SCCVII cells. On the other hand, DC maturation occurred in additional mild HT (mHT: 41˚C for 30 min) with simple HT. To assess whether additional mHT was effective, in vivo combined treatment was performed using tumor-bearing C3H/HeJ mice. A more suppressive effect of tumor growth was observed, and cytotoxic T cell infiltration was significantly increased by adding mHT compared to conventional only simple HT with DCs. These phenomena also occurred in non-treated contralateral tumors as well as treated ones. Our data suggest that the combination of 43˚C preheated simple HT SCCVII tumors and additional 41˚C heat mHT promotes DC maturation, resulting in suppression of tumor growth systemically and lifetime prolongation in mice. A three-step process of additional mHT after local HT and intratumoral immature DC (iDC) injection could be a more effective and novel method for the treatment of SCC.

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June 2011
Volume 25 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Matsumoto, K., Yamamoto, N., Hagiwara, S., Saito, M., Furue, H., Shigetomi, T. ... Ueda, M. (2011). Optimization of hyperthermia and dendritic cell immunotherapy for squamous cell carcinoma. Oncology Reports, 25, 1525-1532. https://doi.org/10.3892/or.2011.1232
MLA
Matsumoto, K., Yamamoto, N., Hagiwara, S., Saito, M., Furue, H., Shigetomi, T., Narita, Y., Mitsudo, K., Tohnai, I., Kobayashi, T., Ueda, M."Optimization of hyperthermia and dendritic cell immunotherapy for squamous cell carcinoma". Oncology Reports 25.6 (2011): 1525-1532.
Chicago
Matsumoto, K., Yamamoto, N., Hagiwara, S., Saito, M., Furue, H., Shigetomi, T., Narita, Y., Mitsudo, K., Tohnai, I., Kobayashi, T., Ueda, M."Optimization of hyperthermia and dendritic cell immunotherapy for squamous cell carcinoma". Oncology Reports 25, no. 6 (2011): 1525-1532. https://doi.org/10.3892/or.2011.1232