Effect of hyperthermia on invasion ability and TGF-β1 expression of breast carcinoma MCF-7 cells

  • Authors:
    • Xiaoxue Xie
    • Xunfan Shao
    • Fuping Gao
    • Hekun Jin
    • Jumei Zhou
    • Lehui Du
    • Yingying Zhang
    • Weiwei Ouyang
    • Xiaowen Wang
    • Lingyun Zhao
    • Xiaodong Zhang
    • Jintian Tang
  • View Affiliations

  • Published online on: March 29, 2011     https://doi.org/10.3892/or.2011.1240
  • Pages: 1573-1579
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Abstract

The present study aimed to investigate heating-induced alterations of breast cancer cell invasion abilities and the potential mechanisms associated with TGF-β1 expression. MCF-7 cells were heated at 43, 45, 47 and 37˚C for 30 min. In vitro cell invasion ability was evaluated by matrigel invasion assay. The activity of matrix metalloproteinase (MMP)-2/9 was investigated by gelatin zymographic assays. Expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) was investigated by immunocytochemistry and RT-PCR. Apoptosis was analysed by flow-cytometry. The invasive potential of MCF-7 cells was reduced by heating, and MMP-2/9 secretion and enzymatic activity were suppressed. Furthermore, VEGF and TGF-β1 mRNA and proteins were suppressed by hyperthermia. These results suggest that down-regulation of the expression of TGF-β1, EGF and MMPs by hyperthermia probably accounts for the inhibition of the invasive abilities of MCF-7 cells.

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June 2011
Volume 25 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xie X, Shao X, Gao F, Jin H, Zhou J, Du L, Zhang Y, Ouyang W, Wang X, Zhao L, Zhao L, et al: Effect of hyperthermia on invasion ability and TGF-β1 expression of breast carcinoma MCF-7 cells. Oncol Rep 25: 1573-1579, 2011
APA
Xie, X., Shao, X., Gao, F., Jin, H., Zhou, J., Du, L. ... Tang, J. (2011). Effect of hyperthermia on invasion ability and TGF-β1 expression of breast carcinoma MCF-7 cells. Oncology Reports, 25, 1573-1579. https://doi.org/10.3892/or.2011.1240
MLA
Xie, X., Shao, X., Gao, F., Jin, H., Zhou, J., Du, L., Zhang, Y., Ouyang, W., Wang, X., Zhao, L., Zhang, X., Tang, J."Effect of hyperthermia on invasion ability and TGF-β1 expression of breast carcinoma MCF-7 cells". Oncology Reports 25.6 (2011): 1573-1579.
Chicago
Xie, X., Shao, X., Gao, F., Jin, H., Zhou, J., Du, L., Zhang, Y., Ouyang, W., Wang, X., Zhao, L., Zhang, X., Tang, J."Effect of hyperthermia on invasion ability and TGF-β1 expression of breast carcinoma MCF-7 cells". Oncology Reports 25, no. 6 (2011): 1573-1579. https://doi.org/10.3892/or.2011.1240