Claudin-11 decreases the invasiveness of bladder cancer cells

  • Authors:
    • Ninaad S. Awsare
    • Tracey A. Martin
    • Mark D. Haynes
    • Philip N. Matthews
    • Wen G. Jiang
  • View Affiliations

  • Published online on: April 4, 2011     https://doi.org/10.3892/or.2011.1244
  • Pages: 1503-1509
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The expression of claudin-11 in benign and malignant bladder tissue and the effect of forced expression of claudin-11 on tight junction function and invasiveness of bladder cancer cells were studied. Claudin-11 expression was tested in bladder cancer cell lines (T24/83, RT 112/84 and EJ138) using reverse transcription-polymerase chain reaction (RT-PCR) and in benign and malignant bladder tissue by quantitative RT-PCR and immunohistochemistry. T24/83 cells were transfected with the pcDNA.1/NT-GFP-TOPO vector containing full-length human claudin-11 sequence. Stable-transfected cells overexpressing claudin-11 (T24Cl-11Ex), wild-type cells (T24WT) and the empty plasmid control clone (T24GFP) were compared using transurothelial resistance (TUR), in vitro adhesion, invasion and growth assays. Claudin-11 was strongly expressed in the non-invasive RT112/84 cell line compared to the invasive T24/83 and EJ138 TCC cell lines. Benign bladder tissue demonstrated equal expression of claudin-11 mRNA as carcinoma, but displayed more intense staining than malignant tissue on immunohistochemistry. Forced-expression of claudin-11 in T24/83 cells was confirmed by PCR, immunoprecipitation and by immunofluorescence, which demonstrated increased perinuclear claudin-11 staining. Forced expression of claudin-11 did not affect TUR (p=0.243), but significantly reduced invasion (p=0.001) while increasing cell matrix adhesion (p=0.001) and growth rates (p=0.001). The greater expression of claudin-11 in benign vs. malignant tissue and non-invasive vs. invasive cell lines, and its effect in reducing bladder cancer cell invasiveness suggests that claudin-11 may have a role in preventing cancer progression and may serve as a therapeutic target in reducing metastasis.

Related Articles

Journal Cover

June 2011
Volume 25 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Awsare, N.S., Martin, T.A., Haynes, M.D., Matthews, P.N., & Jiang, W.G. (2011). Claudin-11 decreases the invasiveness of bladder cancer cells. Oncology Reports, 25, 1503-1509. https://doi.org/10.3892/or.2011.1244
MLA
Awsare, N. S., Martin, T. A., Haynes, M. D., Matthews, P. N., Jiang, W. G."Claudin-11 decreases the invasiveness of bladder cancer cells". Oncology Reports 25.6 (2011): 1503-1509.
Chicago
Awsare, N. S., Martin, T. A., Haynes, M. D., Matthews, P. N., Jiang, W. G."Claudin-11 decreases the invasiveness of bladder cancer cells". Oncology Reports 25, no. 6 (2011): 1503-1509. https://doi.org/10.3892/or.2011.1244