microRNA-21 modulates cell proliferation and sensitivity to doxorubicin in bladder cancer cells

  • Authors:
    • Jun Tao
    • Qiang Lu
    • Deyao Wu
    • Pengchao Li
    • Bin Xu
    • Weijie Qing
    • Meilin Wang
    • Zhengdong Zhang
    • Wei Zhang
  • View Affiliations

  • Published online on: April 4, 2011     https://doi.org/10.3892/or.2011.1245
  • Pages: 1721-1729
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Abstract

Transitional cell carcinomas (TCCs) of the urinary bladder are common malignancies with a high recurrence rate. Since microRNA-21 (miR-21) may contribute to tumorigenesis and chemoresistance in many cancer types, we aimed to investigate its efficacy in TCCs. The expression of miR-21 and its target PTEN was determined by real-time qRT-PCR and western blotting, respectively in tumor tissues as well as adjacent non-tumor mucosa. The effect of miR-21 on cell proliferation and chemosensitivity to doxorubicin were measured using the MTT method. Cell apoptosis induced by doxorubicin was investigated using flow cytometry in the T24 cell line. BCL-2, AKT and pAKT were detected by western blotting for analysis of potential mechanisms. miR-21 was significantly up-regulated in tumor tissues while PTEN was expressed in lower levels compared to non-tumor tissues. A negative correlation between expression of miR-21 and PTEN was established in vivo. Cell proliferation and chemoresistance to doxorubicin were promoted by overexpression of miR-21 in T24 cells. BCL-2 up-regulation could be achieved by miR-21 overexpression, which prevented T24 cells from apoptosis induced by doxorubicin. Furthermore, the miR-21 induced BCL-2 up-regulation could be cancelled by the PI3K inhibitor LY294002. These data verified the oncogenic role of miR-21 in TCCs and may usher in new therapeutic strategies in treating this disease.

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June 2011
Volume 25 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tao J, Lu Q, Wu D, Li P, Xu B, Qing W, Wang M, Zhang Z and Zhang W: microRNA-21 modulates cell proliferation and sensitivity to doxorubicin in bladder cancer cells. Oncol Rep 25: 1721-1729, 2011.
APA
Tao, J., Lu, Q., Wu, D., Li, P., Xu, B., Qing, W. ... Zhang, W. (2011). microRNA-21 modulates cell proliferation and sensitivity to doxorubicin in bladder cancer cells. Oncology Reports, 25, 1721-1729. https://doi.org/10.3892/or.2011.1245
MLA
Tao, J., Lu, Q., Wu, D., Li, P., Xu, B., Qing, W., Wang, M., Zhang, Z., Zhang, W."microRNA-21 modulates cell proliferation and sensitivity to doxorubicin in bladder cancer cells". Oncology Reports 25.6 (2011): 1721-1729.
Chicago
Tao, J., Lu, Q., Wu, D., Li, P., Xu, B., Qing, W., Wang, M., Zhang, Z., Zhang, W."microRNA-21 modulates cell proliferation and sensitivity to doxorubicin in bladder cancer cells". Oncology Reports 25, no. 6 (2011): 1721-1729. https://doi.org/10.3892/or.2011.1245