Screening for serological biomarkers of pancreatic cancer by two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry

  • Authors:
    • Yufeng Wang
    • Yasuhiro Kuramitsu
    • Shigefumi Yoshino
    • Motonari Takashima
    • Xiulian Zhang
    • Tomio Ueno
    • Nobuaki Suzuki
    • Masaaki Oka
    • Kazuyuki Nakamura
  • View Affiliations

  • Published online on: April 21, 2011     https://doi.org/10.3892/or.2011.1278
  • Pages: 287-292
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Abstract

Pancreatic cancer (PC) is one of the most lethal malignant tumors because of late diagnosis and the lack of response to various therapies. To identify potential biomarkers in cancerous serum from early stage PC patients, we carried out two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to compare the serum proteomic profiles from 45 patients with PC and 20 healthy volunteers. Seven spots showed differential expression on 2-DE gels and two up-regulated protein spots were identified by LC-MS/MS as α-1-antitrypsin (AAT). These protein spots were also confirmed by Western blotting. This is the first time that AAT isoforms have been identified as potential serum biomarkers for PC. The serum isoforms of AAT may be clinically useful for PC diagnosis and monitoring.

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July 2011
Volume 26 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang Y, Kuramitsu Y, Yoshino S, Takashima M, Zhang X, Ueno T, Suzuki N, Oka M and Nakamura K: Screening for serological biomarkers of pancreatic cancer by two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry. Oncol Rep 26: 287-292, 2011
APA
Wang, Y., Kuramitsu, Y., Yoshino, S., Takashima, M., Zhang, X., Ueno, T. ... Nakamura, K. (2011). Screening for serological biomarkers of pancreatic cancer by two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry. Oncology Reports, 26, 287-292. https://doi.org/10.3892/or.2011.1278
MLA
Wang, Y., Kuramitsu, Y., Yoshino, S., Takashima, M., Zhang, X., Ueno, T., Suzuki, N., Oka, M., Nakamura, K."Screening for serological biomarkers of pancreatic cancer by two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry". Oncology Reports 26.1 (2011): 287-292.
Chicago
Wang, Y., Kuramitsu, Y., Yoshino, S., Takashima, M., Zhang, X., Ueno, T., Suzuki, N., Oka, M., Nakamura, K."Screening for serological biomarkers of pancreatic cancer by two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry". Oncology Reports 26, no. 1 (2011): 287-292. https://doi.org/10.3892/or.2011.1278