27-Hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype

  • Authors:
    • Cristian G. Torres
    • María E. Ramírez
    • Pamela Cruz
    • María J. Epuñan
    • Luis E. Valladares
    • Walter D. Sierralta
  • View Affiliations

  • Published online on: April 28, 2011     https://doi.org/10.3892/or.2011.1284
  • Pages: 389-397
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Abstract

A decrease in the expression of E-cadherin and β-catenin, paralleling the loss of adherens junction complex, was observed in MCF7 cells exposed for longer than 48 h to 2 µM 27-hydroxycholesterol (27OHC), indicating an epithelial-mesenchymal transition (EMT). Upon removal of 27OHC from the culture medium, the cells released by the exposure of 72 h to the oxysterol grew as loosely packed cell groups. In these cells, accumulation of E-cadherin and β-catenin in the cytoplasm and the prolonged expression of epidermal growth factor receptor 2 (EGFR2/neu) in the plasma membrane were observed, suggesting that the acquired phenotype was related to the expression of this tyrosine kinase-growth factor receptor. The results presented here are discussed on the basis of the claimed relationship between 27OHC, hypercholesterolemia, macrophage infiltration and therapy-resistant ERα+ breast cancer incidence.

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August 2011
Volume 26 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Torres CG, Ramírez ME, Cruz P, Epuñan MJ, Valladares LE and Sierralta WD: 27-Hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype. Oncol Rep 26: 389-397, 2011
APA
Torres, C.G., Ramírez, M.E., Cruz, P., Epuñan, M.J., Valladares, L.E., & Sierralta, W.D. (2011). 27-Hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype. Oncology Reports, 26, 389-397. https://doi.org/10.3892/or.2011.1284
MLA
Torres, C. G., Ramírez, M. E., Cruz, P., Epuñan, M. J., Valladares, L. E., Sierralta, W. D."27-Hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype". Oncology Reports 26.2 (2011): 389-397.
Chicago
Torres, C. G., Ramírez, M. E., Cruz, P., Epuñan, M. J., Valladares, L. E., Sierralta, W. D."27-Hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype". Oncology Reports 26, no. 2 (2011): 389-397. https://doi.org/10.3892/or.2011.1284