Overexpression of Nampt in gastric cancer and chemopotentiating effects of the Nampt inhibitor FK866 in combination with fluorouracil

  • Authors:
    • Tie-Qiang Bi
    • Xiang-Ming Che
    • Xin-Hua Liao
    • Dan-Jie Zhang
    • Hou-Long Long
    • Hai-Jun Li
    • Wei Zhao
  • View Affiliations

  • Published online on: July 4, 2011     https://doi.org/10.3892/or.2011.1378
  • Pages:1251-1257
0

Abstract

Nicotinamide phosphoribosyltransferase (Nampt), an enzyme involved in the NAD+ salvage pathway, is over­expressed and important in the carcinogenesis in several types of cancers. The expression of Nampt and its role in gastric cancer remain largely unknown. In this study, using real-time PCR and Western blotting we found that Nampt was overexpressed at the mRNA and protein levels, respectively, in established gastric cancer cells and human gastric cancer tissues. The specific Nampt inhibitor FK866 repressed gastric cancer cell proliferation, as assessed by MTT assay. Using transwell and soft agar clonogenic assays, we also found that FK866 suppressed gastric cancer cell migration and anchorage-independent growth, respectively. These inhibitory effects of FK866 were accompanied by significantly decreased expression of VEGF, MMP2, MMP9 and NF-κB. As determined by MTT assay and flow cytometry, FK866 also increased the chemo­sensitivity of gastric cancer cells to fluorouracil by greater inhibition of cell proliferation and the induction of apoptosis. Our findings indicate that Nampt may be a new therapeutic target for gastric cancer.

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November 2011
Volume 26 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
Ranked #31/217 Oncology
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APA
Bi, T., Che, X., Liao, X., Zhang, D., Long, H., Li, H., & Zhao, W. (2011). Overexpression of Nampt in gastric cancer and chemopotentiating effects of the Nampt inhibitor FK866 in combination with fluorouracil. Oncology Reports, 26, 1251-1257. https://doi.org/10.3892/or.2011.1378
MLA
Bi, T., Che, X., Liao, X., Zhang, D., Long, H., Li, H., Zhao, W."Overexpression of Nampt in gastric cancer and chemopotentiating effects of the Nampt inhibitor FK866 in combination with fluorouracil". Oncology Reports 26.5 (2011): 1251-1257.
Chicago
Bi, T., Che, X., Liao, X., Zhang, D., Long, H., Li, H., Zhao, W."Overexpression of Nampt in gastric cancer and chemopotentiating effects of the Nampt inhibitor FK866 in combination with fluorouracil". Oncology Reports 26, no. 5 (2011): 1251-1257. https://doi.org/10.3892/or.2011.1378