Role of GOLPH3 and GOLPH3L in the proliferation of human rhabdomyosarcoma

  • Authors:
    • Osamu Kunigou
    • Hiroko Nagao
    • Naoya Kawabata
    • Yasuhiro Ishidou
    • Satoshi Nagano
    • Shingo Maeda
    • Setsuro Komiya
    • Takao Setoguchi
  • View Affiliations

  • Published online on: August 5, 2011     https://doi.org/10.3892/or.2011.1413
  • Pages: 1337-1342
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Abstract

GOLPH3 was originally identified by proteomic analyses of Golgi proteins localized in the trans-Golgi network. Recently, it was reported that GOLPH3 is up-regulated in various types of malignancies, including melanoma, colon cancer and lung cancer. However, the mechanism through which GOLPH3 is involved in the pathogenesis of rhabdomyosarcoma remains unidentified. In order to explore the function of GOLPH3 and its isoform, GOLPH3L, in the pathogenesis of rhabdomyosarcoma, we investigated the expression and knockdown effects of GOLPH3 and GOLPH3L in human rhabdomyosarcoma. Western blot analysis and real-time PCR revealed that human rhabdomyosarcoma cell lines and biopsy specimens exhibited an increased expression of GOLPH3 and GOLPH3L. GOLPH3 and GOLPH3L knockdown by siRNA prevented the proliferation of human rhabdomyosarcoma cell lines. In addition, double-knockdown of GOLPH3 and GOLPH3L also prevented the proliferation of rhabdomyosarcoma cell lines. Our findings improve the understanding of rhabdomyosarcoma pathogenesis and suggest that the knockdown of GOLPH3 or GOLPH3L may be an effective treatment for rhabdomyosarcoma.

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November 2011
Volume 26 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kunigou O, Nagao H, Kawabata N, Ishidou Y, Nagano S, Maeda S, Komiya S and Setoguchi T: Role of GOLPH3 and GOLPH3L in the proliferation of human rhabdomyosarcoma. Oncol Rep 26: 1337-1342, 2011
APA
Kunigou, O., Nagao, H., Kawabata, N., Ishidou, Y., Nagano, S., Maeda, S. ... Setoguchi, T. (2011). Role of GOLPH3 and GOLPH3L in the proliferation of human rhabdomyosarcoma. Oncology Reports, 26, 1337-1342. https://doi.org/10.3892/or.2011.1413
MLA
Kunigou, O., Nagao, H., Kawabata, N., Ishidou, Y., Nagano, S., Maeda, S., Komiya, S., Setoguchi, T."Role of GOLPH3 and GOLPH3L in the proliferation of human rhabdomyosarcoma". Oncology Reports 26.5 (2011): 1337-1342.
Chicago
Kunigou, O., Nagao, H., Kawabata, N., Ishidou, Y., Nagano, S., Maeda, S., Komiya, S., Setoguchi, T."Role of GOLPH3 and GOLPH3L in the proliferation of human rhabdomyosarcoma". Oncology Reports 26, no. 5 (2011): 1337-1342. https://doi.org/10.3892/or.2011.1413