Induction of apoptosis by evodiamine involves both activation of mitotic arrest and mitotic slippage

  • Authors:
    • Li-Hong Zhu
    • Wei Bi
    • Xiao-Dong Liu
    • Jie-Fen Li
    • Ying-Ya Wu
    • Biao-Yan Du
    • Yu-Hui Tan
  • View Affiliations

  • Published online on: September 1, 2011     https://doi.org/10.3892/or.2011.1444
  • Pages: 1447-1455
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Abstract

Evodiamine (Evo) is an indole quinazoline alkaloid isolated from the fruit of Evodia rutaecarpa Bentham. Previous studies have shown that Evo exhibits anti-proliferative anti-tumor activities in several cancer types, but its target(s) and underlying mechanism(s) of action remain unclear. In the present study, we sought to establish a cell synchronization model in order to examine the anti-proliferative and apoptotic mechanisms of Evo in the human gastric cancer cell line SGC-7901. In addition, we transfected these cells with full-length or non-degradable (ND) cyclinB1 to evaluate the relationship between the induction of apoptosis and activation of mitotic arrest and mitotic slippage by Evo. Our results demonstrated that Evo markedly inhibited cell growth and was cytotoxic to SGC-7901 cells. Furthermore, transient Evo treatment (<16 h) caused reversible mitotic arrest, but sustained mitotic arrest was required to initiate apoptosis. The time required to reverse the apoptotic effects of Evo was between 16 and 20 h. We also demonstrated that promotion of mitotic slippage by a CDK1 inhibitor enhanced apoptosis. Furthermore, we evaluated the effect of delaying mitotic slippage by overexpressing ND cyclinB1, which delayed apoptosis. In conclusion, these results indicate that Evo-induced apoptosis is associated with mitotic arrest and subsequent mitotic slippage, which may underlie the actions of Evo in the treatment and prevention of cancer.

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December 2011
Volume 26 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhu L, Bi W, Liu X, Li J, Wu Y, Du B and Tan Y: Induction of apoptosis by evodiamine involves both activation of mitotic arrest and mitotic slippage. Oncol Rep 26: 1447-1455, 2011
APA
Zhu, L., Bi, W., Liu, X., Li, J., Wu, Y., Du, B., & Tan, Y. (2011). Induction of apoptosis by evodiamine involves both activation of mitotic arrest and mitotic slippage. Oncology Reports, 26, 1447-1455. https://doi.org/10.3892/or.2011.1444
MLA
Zhu, L., Bi, W., Liu, X., Li, J., Wu, Y., Du, B., Tan, Y."Induction of apoptosis by evodiamine involves both activation of mitotic arrest and mitotic slippage". Oncology Reports 26.6 (2011): 1447-1455.
Chicago
Zhu, L., Bi, W., Liu, X., Li, J., Wu, Y., Du, B., Tan, Y."Induction of apoptosis by evodiamine involves both activation of mitotic arrest and mitotic slippage". Oncology Reports 26, no. 6 (2011): 1447-1455. https://doi.org/10.3892/or.2011.1444