MiR-25 regulates apoptosis by targeting Bim in human ovarian cancer

  • Authors:
    • Haiyan Zhang
    • Zhi Zuo
    • Xin Lu
    • Li Wang
    • Haiyan Wang
    • Zhiling Zhu
  • View Affiliations

  • Published online on: Tuesday, November 8, 2011
  • Pages: 594-598 DOI: 10.3892/or.2011.1530

Abstract

MicroRNAs (miRNAs) are emerging as a class of small regulatory RNAs whose alterations are implicated in the initiation and progression of human cancers. Our study showed that miR-25 was highly expressed both in clinical ovarian cancer samples and cell lines. Down-regulation of miR-25 in ovarian cancer cells induced apoptosis whereas overexpression of miR-25 enhanced cell proliferation. The effects of miR-25 abrogation were partly mediated by the intrinsic apoptosis pathway. Many pro-apoptotic proteins such as Bim, Bax and caspase-3 were up-regulated after transfection. Furthermore, luciferase assays demonstrated that Bim was the direct target of miR-25. Introducing Bim cDNA without 3'UTR abrogated miR-25-induced cell survival. Finally, there was an inverse relationship between Bim and miR-25 expression in ovarian cancer tissues. Taken together, these data indicate that miR-25 directly regulates apoptosis by targeting Bim in ovarian cancer and that miR-25 could be a potential therapeutic target for ovarian cancer intervention.

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February 2012
Volume 27 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

2015 Impact Factor: 2.486
Ranked #31/211 Oncology
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APA
Zhang, H., Zuo, Z., Lu, X., Wang, L., Wang, H., & Zhu, Z. (2012). MiR-25 regulates apoptosis by targeting Bim in human ovarian cancer. Oncology Reports, 27, 594-598. http://dx.doi.org/10.3892/or.2011.1530
MLA
Zhang, H., Zuo, Z., Lu, X., Wang, L., Wang, H., Zhu, Z."MiR-25 regulates apoptosis by targeting Bim in human ovarian cancer". Oncology Reports 27.2 (2012): 594-598.
Chicago
Zhang, H., Zuo, Z., Lu, X., Wang, L., Wang, H., Zhu, Z."MiR-25 regulates apoptosis by targeting Bim in human ovarian cancer". Oncology Reports 27, no. 2 (2012): 594-598. http://dx.doi.org/10.3892/or.2011.1530