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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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February 2012 Volume 27 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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February 2012 Volume 27 Issue 2

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Article

shRNA against CD44 inhibits cell proliferation, invasion and migration, and promotes apoptosis of colon carcinoma cells

  • Authors:
    • Yeon Sun Park
    • Jung Wook Huh
    • Jae Hyuk Lee
    • Hyeong Rok Kim

  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Chonnam National University Medical School and Hwasun Hospital, Hwasun-gun, Gwangju 519-809, Republic of Korea, Department of Surgery, Chonnam National University Medical School, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Gwangju 519-809, Republic of Korea
  • Pages: 339-346
    |
    Published online on: November 8, 2011
       https://doi.org/10.3892/or.2011.1532
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Abstract

CD44 is a causal factor for tumor invasion, metastasis and acquisition of resistance to apoptosis. CD44 knockdown using inducible short hairpin RNA (shRNA) significantly reduces cell growth and invasion. Short hairpin RNA against CD44 and pGFP-V-RS-vector was used for knockdown of CD44 expression in SW620 colon cancer cells. Cell growth, invasion and migration assay, immunofluorescence for β-catenin expression and western blotting for Wnt signaling molecules were analyzed. Cell cycle analysis and western blot analysis for apoptotic molecules were evaluated. Short hairpin RNA against CD44 reduced the expression of CD44. Cell proliferation, migration and invasion were markedly inhibited and apoptosis was increased in shRNA CD44-transfected cells. Knockdown of CD44 decreased the phosphorylation of PDK1, Akt and GSK3β, and β-catenin levels. Decreased phosphorylated Akt led to an increase in phosphorylated FoxO1 and induced cell cycle arrest in the G0-G1 phase and a decrease in the S phase. The levels of Bcl-2 and Bcl-xL expression were down-regulated, while the levels of BAX expression and cleaved caspase-3, -8 and -9 were increased. CD44 knockdown by way of shRNA inhibited cell proliferation and induced cell apoptosis. This can be used as a therapeutic intervention with the anti-survival/pro-apoptotic machinery in human colon cancer.

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Copy and paste a formatted citation
Spandidos Publications style
Park YS, Huh JW, Lee JH and Kim HR: shRNA against CD44 inhibits cell proliferation, invasion and migration, and promotes apoptosis of colon carcinoma cells. Oncol Rep 27: 339-346, 2012.
APA
Park, Y.S., Huh, J.W., Lee, J.H., & Kim, H.R. (2012). shRNA against CD44 inhibits cell proliferation, invasion and migration, and promotes apoptosis of colon carcinoma cells. Oncology Reports, 27, 339-346. https://doi.org/10.3892/or.2011.1532
MLA
Park, Y. S., Huh, J. W., Lee, J. H., Kim, H. R."shRNA against CD44 inhibits cell proliferation, invasion and migration, and promotes apoptosis of colon carcinoma cells". Oncology Reports 27.2 (2012): 339-346.
Chicago
Park, Y. S., Huh, J. W., Lee, J. H., Kim, H. R."shRNA against CD44 inhibits cell proliferation, invasion and migration, and promotes apoptosis of colon carcinoma cells". Oncology Reports 27, no. 2 (2012): 339-346. https://doi.org/10.3892/or.2011.1532
Copy and paste a formatted citation
x
Spandidos Publications style
Park YS, Huh JW, Lee JH and Kim HR: shRNA against CD44 inhibits cell proliferation, invasion and migration, and promotes apoptosis of colon carcinoma cells. Oncol Rep 27: 339-346, 2012.
APA
Park, Y.S., Huh, J.W., Lee, J.H., & Kim, H.R. (2012). shRNA against CD44 inhibits cell proliferation, invasion and migration, and promotes apoptosis of colon carcinoma cells. Oncology Reports, 27, 339-346. https://doi.org/10.3892/or.2011.1532
MLA
Park, Y. S., Huh, J. W., Lee, J. H., Kim, H. R."shRNA against CD44 inhibits cell proliferation, invasion and migration, and promotes apoptosis of colon carcinoma cells". Oncology Reports 27.2 (2012): 339-346.
Chicago
Park, Y. S., Huh, J. W., Lee, J. H., Kim, H. R."shRNA against CD44 inhibits cell proliferation, invasion and migration, and promotes apoptosis of colon carcinoma cells". Oncology Reports 27, no. 2 (2012): 339-346. https://doi.org/10.3892/or.2011.1532
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