Open Access

Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma

  • Authors:
    • Chan Yong Kim
    • Woon Yong Jung
    • Hyun Joo Lee
    • Han Kyeom Kim
    • Aeree Kim
    • Bong Kyung Shin
  • View Affiliations

  • Published online on: November 10, 2011     https://doi.org/10.3892/or.2011.1545
  • Pages: 608-620
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Abstract

The study of tumor biomarkers was gradually facilitated by the adoption of proteomic strategies due to less invasiveness and higher sensitivity. Colorectal cancer is one of the most commonly occurring cancers worldwide and its incidence has markedly increased in Korea. While the adoption of proteomic strategies facilitated the study of tumor biomarkers, to date, no common agreement has been derived from proteomic investigations regarding tumor markers of colorectal cancer. This study was designed to find molecules differentially expressed in colorectal cancer compared to non-tumor mucosa. Four colorectal adenocarcinoma and corresponding non-tumor tissue samples were analyzed to find previously unknown proteins via two-dimensional electrophoresis and MALDI-TOF/MS spectrometry. Western blot assays and tissue microarray (TMA) immunohistochemistry were performed to validate the identified proteins. Among the twelve up-regulated and one down-regulated proteins identified, moesin, cytokeratin (KRT) 17 and carbonic anhydrase I were validated by western blot analysis and/or immunohistochemistry. On immunohistochemistry, both moesin and KRT17 demonstrated a tendency of increased expression as pT stage advanced. Both moesin and KRT17 were not expressed in normal colorectal epithelium. These two proteins may play a role in cancer invasion and/or metastasis in colorectal carcinoma, and could be candidate biomarkers for the diagnosis and prognosis of colorectal cancer.

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March 2012
Volume 27 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kim CY, Jung WY, Lee HJ, Kim HK, Kim A and Shin BK: Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma. Oncol Rep 27: 608-620, 2012
APA
Kim, C.Y., Jung, W.Y., Lee, H.J., Kim, H.K., Kim, A., & Shin, B.K. (2012). Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma. Oncology Reports, 27, 608-620. https://doi.org/10.3892/or.2011.1545
MLA
Kim, C. Y., Jung, W. Y., Lee, H. J., Kim, H. K., Kim, A., Shin, B. K."Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma". Oncology Reports 27.3 (2012): 608-620.
Chicago
Kim, C. Y., Jung, W. Y., Lee, H. J., Kim, H. K., Kim, A., Shin, B. K."Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma". Oncology Reports 27, no. 3 (2012): 608-620. https://doi.org/10.3892/or.2011.1545