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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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May 2012 Volume 27 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

Plasmid DNA introduced into cultured cells with diagnostic ultrasound

  • Authors:
    • Minoru Tomizawa
    • Fuminobu Shinozaki
    • Takao Sugiyama
    • Shigenori Yamamoto
    • Makoto Sueishi
    • Takanobu Yoshida
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, Yotsukaido City, Chiba 284-0003, Japan
  • Pages: 1360-1364
    |
    Published online on: January 19, 2012
       https://doi.org/10.3892/or.2012.1643
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Abstract

The usefulness of diagnostic ultrasound in gene transfer was investigated. The hepatocellular carcinoma cell lines PRL/PRF/5 and Hep3B, and the pancreatic carcinoma Panc-1 cells were transfected with lipofectin or irradiated with a linear probe with a frequency of 8 MHz at a mechanical index of 0.4 through the bottom of the plates for 5 min using diagnostic ultrasound (US) with pEGFP-N1 [green fluorescent protein (GFP) expression plasmid], and observed under fluorescence microscopy 48 h later. The cell lines were transfected or irradiated with US with pGL3 control (luciferase reporter plasmid), and a luciferase assay (48 h later) or an MTS assay (72 h later) were performed. Although signals of GFP were observed in cells with US, the transfection efficiencies of US were lower than those of transfection. Luciferase activities of cells with US were higher than those of non-irradiated or transfected cells, but lower than those of transfection. Cell viability with US did not change.

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Copy and paste a formatted citation
Spandidos Publications style
Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Plasmid DNA introduced into cultured cells with diagnostic ultrasound. Oncol Rep 27: 1360-1364, 2012.
APA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., & Yoshida, T. (2012). Plasmid DNA introduced into cultured cells with diagnostic ultrasound. Oncology Reports, 27, 1360-1364. https://doi.org/10.3892/or.2012.1643
MLA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Plasmid DNA introduced into cultured cells with diagnostic ultrasound". Oncology Reports 27.5 (2012): 1360-1364.
Chicago
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Plasmid DNA introduced into cultured cells with diagnostic ultrasound". Oncology Reports 27, no. 5 (2012): 1360-1364. https://doi.org/10.3892/or.2012.1643
Copy and paste a formatted citation
x
Spandidos Publications style
Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Plasmid DNA introduced into cultured cells with diagnostic ultrasound. Oncol Rep 27: 1360-1364, 2012.
APA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., & Yoshida, T. (2012). Plasmid DNA introduced into cultured cells with diagnostic ultrasound. Oncology Reports, 27, 1360-1364. https://doi.org/10.3892/or.2012.1643
MLA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Plasmid DNA introduced into cultured cells with diagnostic ultrasound". Oncology Reports 27.5 (2012): 1360-1364.
Chicago
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Plasmid DNA introduced into cultured cells with diagnostic ultrasound". Oncology Reports 27, no. 5 (2012): 1360-1364. https://doi.org/10.3892/or.2012.1643
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