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Print ISSN: 1021-335X Online ISSN: 1791-2431
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December 2012 Volume 28 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

1,25-Dihydroxyvitamin D3 inhibits growth of the breast cancer cell line MCF-7 and downregulates cytochrome P4501B1 through the COX-2/PGE2 pathway

  • Authors:
    • Lei Yuan
    • Rong Jiang
    • Yingχue Yang
    • Songtao Ding
    • Huayu Deng
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, P.R. China, Institute of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 2131-2137
    |
    Published online on: September 12, 2012
       https://doi.org/10.3892/or.2012.2031
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Abstract

Cytochrome P4501B1 (CYP1B1) is responsible for tumor progression in estrogen receptor-positive breast cancer due to its key role in estrogen metabolism, which is upregulated by PGE2, the main product of COX-2 that is found to be overexpressed in many breast tumors. Previous studies reported that inhibition of the COX-2/PGE2 pathway, by 1,25-dihydroxyvitamin D3 in MCF-7 breast cancer cells. The aim of this study was to investigate if the CYP1B1 protein expression shows covariation with the COX-2 and phosphorylated ERα (p-ERα) in human breast cancer. We also investigated whether 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] downregulates CYP1B1 via the COX-2/PGE2 pathway in MCF-7 cells. We analyzed the expression of COX-2, p-ERα and CYP1B1 using an immunohistochemical staining assay. In the present study, COX-2 was correlated to p-ERα (P<0.001) and CYP1B1 (P=0.001), p-ERα was correlated to CYP1B1 (P=0.012). We assessed the effects of 1,25(OH)2D3 on MCF-7 cells. 1,25(OH)2D3 treatment inhibited MCF-7 cell growth in a time-and dose-dependent manner; the cell cycle was arrested in the G0/G1 phase. Treatment with 100 nmol/l 1,25(OH)2D3 for 72 h significantly decreased the expression of COX-2 mRNA in MCF-7 cells (P<0.05), decreased the levels of PGE2 in cell culture supernatant (P<0.01), and downregul­ated p-ERK, p-ERα and CYP1B1 protein expression (P<0.05). Taken together, these results suggest that the COX-2/PGE2 pathway positively regulates the expression of CYP1B1 in breast cancer. 1,25-Dihydroxyvitamin D3 inhibits the growth of MCF-7 cells and downregulates CYP1B1 mediated by the COX-2/PGE2 pathway.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

2 

Bertone-Johnson ER: Vitamin D and breast cancer. Ann Epidemiol. 19:462–467. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Bertone-Johnson ER, Chen WY, Holick MF, et al: Plasma 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D and risk of breast cancer. Cancer Epidemiol Biomarkers Prev. 14:1991–1997. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Krishnan AV, Swami S and Feldman D: Vitamin D and breast cancer: inhibition of estrogen synthesis and signaling. J Steroid Biochem Mol Biol. 121:343–348. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Subbaramaiah K, Hudis C, Chang SH, Hla T and Dannenberg AJ: EP2 and EP4 receptors regulate aromatase expression in human adipocytes and breast cancer cells. Evidence of a BRCA1 and p300 exchange. J Biol Chem. 283:3433–3444. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Han EH, Kim HG, Hwang YP, Song GY and Jeong HG: Prostaglandin E2 induces CYP1B1 expression via ligand-independent activation of the ERα pathway in human breast cancer cells. Toxicol Sci. 114:204–216. 2010.PubMed/NCBI

7 

Han D, Denison MS, Tachibana H and Yamada K: Effects of estrogenic compounds on immunoglobulin production by mouse splenocytes. Biol Pharm Bull. 25:1263–1267. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Colotta F, Allavena P, Sica A, Garlanda C and Mantovani A: Cancer-related inflammation, the seventh hallmark of cancer: links to genetic instability. Carcinogenesis. 30:1073–1081. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Ristimaki A, Sivula A, Lundin J, et al: Prognostic significance of elevated cyclooxygenase-2 expression in breast cancer. Cancer Res. 62:632–635. 2002.PubMed/NCBI

10 

Gunnarsson C, Jansson A, Holmlund B, et al: Expression of COX-2 and steroid converting enzymes in breast cancer. Oncol Rep. 16:219–224. 2006.PubMed/NCBI

11 

Krishnan AV, Shinghal R, Raghavachari N, Brooks JD, Peehl DM and Feldman D: Analysis of vitamin D-regulated gene expression in LNCaP human prostate cancer cells using cDNA microarrays. Prostate. 59:243–251. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Krishnan AV, Swami S, Peng L, Wang J, Moreno J and Feldman D: Tissue-selective regulation of aromatase expression by calcitriol: implications for breast cancer therapy. Endocrinology. 151:32–42. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Moreno J, Krishnan AV, Swami S, Nonn L, Peehl DM and Feldman D: Regulation of prostaglandin metabolism by calcitriol attenuates growth stimulation in prostate cancer cells. Cancer Res. 65:7917–7925. 2005.PubMed/NCBI

14 

Greenhough A, Smartt HJ, Moore AE, et al: The COX-2/PGE2 pathway: key roles in the hallmarks of cancer and adaptation to the tumour microenvironment. Carcinogenesis. 30:377–386. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Kushner PJ, Agard DA, Greene GL, et al: Estrogen receptor pathways to AP-1. J Steroid Biochem Mol Biol. 74:311–317. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Feng W, Webb P, Nguyen P, et al: Potentiation of estrogen receptor activation function 1 (AF-1) by Src/JNK through a serine 118-independent pathway. Mol Endocrinol. 15:32–45. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Chen D, Pace PE, Coombes RC and Ali S: Phosphorylation of human estrogen receptor α by protein kinase A regulates dimerization. Mol Cell Biol. 19:1002–1015. 1999.

18 

Cavalieri E, Chakravarti D, Guttenplan J, et al: Catechol estrogen quinones as initiators of breast and other human cancers: implications for biomarkers of susceptibility and cancer prevention. Biochim Biophys Acta. 1766:63–78. 2006.PubMed/NCBI

19 

Bruno RD and Njar VC: Targeting cytochrome P450 enzymes: a new approach in anti-cancer drug development. Bioorg Med Chem. 15:5047–5060. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Tsuchiya Y, Nakajima M, Kyo S, Kanaya T, Inoue M and Yokoi T: Human CYP1B1 is regulated by estradiol via estrogen receptor. Cancer Res. 64:3119–3125. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yuan L, Jiang R, Yang Y, Ding S and Deng H: 1,25-Dihydroxyvitamin D3 inhibits growth of the breast cancer cell line MCF-7 and downregulates cytochrome P4501B1 through the COX-2/PGE2 pathway. Oncol Rep 28: 2131-2137, 2012.
APA
Yuan, L., Jiang, R., Yang, Y., Ding, S., & Deng, H. (2012). 1,25-Dihydroxyvitamin D3 inhibits growth of the breast cancer cell line MCF-7 and downregulates cytochrome P4501B1 through the COX-2/PGE2 pathway. Oncology Reports, 28, 2131-2137. https://doi.org/10.3892/or.2012.2031
MLA
Yuan, L., Jiang, R., Yang, Y., Ding, S., Deng, H."1,25-Dihydroxyvitamin D3 inhibits growth of the breast cancer cell line MCF-7 and downregulates cytochrome P4501B1 through the COX-2/PGE2 pathway". Oncology Reports 28.6 (2012): 2131-2137.
Chicago
Yuan, L., Jiang, R., Yang, Y., Ding, S., Deng, H."1,25-Dihydroxyvitamin D3 inhibits growth of the breast cancer cell line MCF-7 and downregulates cytochrome P4501B1 through the COX-2/PGE2 pathway". Oncology Reports 28, no. 6 (2012): 2131-2137. https://doi.org/10.3892/or.2012.2031
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan L, Jiang R, Yang Y, Ding S and Deng H: 1,25-Dihydroxyvitamin D3 inhibits growth of the breast cancer cell line MCF-7 and downregulates cytochrome P4501B1 through the COX-2/PGE2 pathway. Oncol Rep 28: 2131-2137, 2012.
APA
Yuan, L., Jiang, R., Yang, Y., Ding, S., & Deng, H. (2012). 1,25-Dihydroxyvitamin D3 inhibits growth of the breast cancer cell line MCF-7 and downregulates cytochrome P4501B1 through the COX-2/PGE2 pathway. Oncology Reports, 28, 2131-2137. https://doi.org/10.3892/or.2012.2031
MLA
Yuan, L., Jiang, R., Yang, Y., Ding, S., Deng, H."1,25-Dihydroxyvitamin D3 inhibits growth of the breast cancer cell line MCF-7 and downregulates cytochrome P4501B1 through the COX-2/PGE2 pathway". Oncology Reports 28.6 (2012): 2131-2137.
Chicago
Yuan, L., Jiang, R., Yang, Y., Ding, S., Deng, H."1,25-Dihydroxyvitamin D3 inhibits growth of the breast cancer cell line MCF-7 and downregulates cytochrome P4501B1 through the COX-2/PGE2 pathway". Oncology Reports 28, no. 6 (2012): 2131-2137. https://doi.org/10.3892/or.2012.2031
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