Upregulation of Mad2 facilitates in vivo and in vitro osteosarcoma progression

Yu,Ling; Liu,Shiqing; Guo,Weichun; Zhang,Bo; Liang,Yi; Feng,Qisheng

doi:10.3892/or.2012.2032

Upregulation of Mad2 facilitates in vivo and in vitro osteosarcoma progression

Authors:
- Ling Yu
- Shiqing Liu
- Weichun Guo
- Bo Zhang
- Yi Liang
- Qisheng Feng
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Affiliations: Department of Orthopedics, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R. China, State Key Laboratory of Oncology in Southern China and the Department of Experimental Research, Sun Yat-Sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China
Published online on: September 17, 2012 https://doi.org/10.3892/or.2012.2032
Pages: 2170-2176

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Abstract

It has been reported that overexpression of Mad2 in transgenic mice leads to a wide variety of tumors, and Mad2 overexpression causes lung tumor relapse after oncogene withdrawal. In a previous study we demonstrated that Mad2 is abnormally upregulated in human osteosarcoma, however, the underlying mechanisms remain unknown. In this study, we found that transient Mad2 overexpression is sufficient to cause early dyscrasia and decreased survival in a xenotransplantation osteosarcoma mouse model, and Mad2 overexpression is associated with increased invasiveness and pulmonary metastasis. We also found that upregulation of Mad2 was accompanied by enhanced capability to self-renew. Our data validate the correlation between upregulation of Mad2 and osteosarcoma advancement, and that the underlying mechanisms involve the increase of invasiveness and cancer stem cell properties.

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