Open Access

A new purification method for enhancing the immunogenicity of heat shock protein 70-peptide complexes

  • Authors:
    • Yanwei Gao
    • Xia Chen
    • Weishi Gao
    • Yong Yang
    • Hulin Ma
    • Xinjun Ren
  • View Affiliations

  • Published online on: September 21, 2012     https://doi.org/10.3892/or.2012.2051
  • Pages: 1977-1983
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

When purified from a tumor, certain heat shock protein 70 (HSP70)-peptide complexes (PCs) can function as effective vaccines against the tumor from which the complexes were isolated. The immunogenic mechanisms of HSP70 preparations imply that tumor-derived HSP70-PCs exhibit antigens associated with antigen-presenting cells such as dendritic cells (DCs), inducing antigen-specific cytotoxic CD8+ T cells. However, some important membrane-resident tumor-associated peptides, such as the HER-2/neu (c-erbB2) oncogenic protein, cannot be purified from HSP70 by traditional methods. In the present study, a new approach for the purification of HSP70-PCs from HER-2-overexpressing breast cancer cells was established. The detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) was used to obtain more effectual tumor peptides. The new purified product was named HSP70-HER-2-PC, and its immunological activities were determined. Traditionally purified HSP70-PCs (without CHAPS) and recombinant human HSP70-HER-2 protein complexes (recombined in vitro) were used as controls. These three HSP70-associated tumor antigenic complex pulsed dendritic cells (DCs) were used to stimulate an antitumor response. The mature DCs pulsed with HSP70-HER-2-PCs stimulated autologous T cells to secrete higher levels of type I cytokine compared to the two control groups. Moreover, DCs pulsed with HSP70-HER-2-PCs induced the most specific CD8+ T cells that specifically killed the same tumor cells. These findings provide a basis for new approaches in enhancing HSP70-based immunotherapy for HER-2-associated or other membrane antigenic peptide-related cancers.
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December 2012
Volume 28 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Gao Y, Chen X, Gao W, Yang Y, Ma H and Ren X: A new purification method for enhancing the immunogenicity of heat shock protein 70-peptide complexes. Oncol Rep 28: 1977-1983, 2012.
APA
Gao, Y., Chen, X., Gao, W., Yang, Y., Ma, H., & Ren, X. (2012). A new purification method for enhancing the immunogenicity of heat shock protein 70-peptide complexes. Oncology Reports, 28, 1977-1983. https://doi.org/10.3892/or.2012.2051
MLA
Gao, Y., Chen, X., Gao, W., Yang, Y., Ma, H., Ren, X."A new purification method for enhancing the immunogenicity of heat shock protein 70-peptide complexes". Oncology Reports 28.6 (2012): 1977-1983.
Chicago
Gao, Y., Chen, X., Gao, W., Yang, Y., Ma, H., Ren, X."A new purification method for enhancing the immunogenicity of heat shock protein 70-peptide complexes". Oncology Reports 28, no. 6 (2012): 1977-1983. https://doi.org/10.3892/or.2012.2051