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December 2012 Volume 28 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line

  • Authors:
    • Yantian Fang
    • Jianbin Xiang
    • Zongyou Chen
    • Xiaodong  Gu
    • Zhengyang Li
    • Feng Tang
    • Zhongwen Zhou
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China, Department of General Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China, Department of Pathology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China
  • Pages: 2115-2124
    |
    Published online on: September 24, 2012
       https://doi.org/10.3892/or.2012.2054
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Abstract

Colorectal cancer (CRC) is one of the major causes of cancer-related mortality worldwide. Recent studies revealed that there is a relationship between CRC occurrence and microRNA (miRNA) function. Stem cells are a type of cells that have the ability to self-renew and to proliferate extensively while maintaining the undifferentiated state. Cancer stem cells (CSCs) are closely linked to tumor recurrence and metastasis. To this end, we evaluated the miRNA expression differences between colon CSCs and non-stem cells using the SW1116 cell line, to determine the relationship between tumor stem cells and tumor biological behavior. We isolated populations of colon CSCs with the CD133+/CD44+ and CD133-/CD44- surface phenotype from a human SW1116 colon adenocarcinoma cell line using flow cytometry. The expression of miRNA and mRNA of both sets of cells was examined with miRNA and mRNA arrays. Bioinformatic methods were used to analyze microarray results. We completed gene ontology analysis, pathway analysis, miRNA target gene prediction with databases. We identified a colon stem cell miRNA expression profile comprising 31 upregulated and 31 downregulated miRNAs, such as miR29a, miR29b, miR449b and miR4524. Some of these differentially expressed miRNAs may be involved in the regulation of stem cell differentiation. Gene ontology and pathway analyses showed that the differences are closely related to the function of the cell cycle, cell differentiation, signaling pathway, cytoskeletal proteins and cell-matrix adhesion in colon cancer stem cells. We found that miRNAs play an important role in regulating the expression of colon CSC characteristics. By regulating the expression of CSC signaling pathways, cytoskeleton and membrane proteins, miRNAs give tumor stem cells the macrobiological behavior of recurrence and metastasis. This study provides a new perspective on CRC metastasis and recurrence.
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Copy and paste a formatted citation
Spandidos Publications style
Fang Y, Xiang J, Chen Z, Gu X, Li Z, Tang F and Zhou Z: miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line. Oncol Rep 28: 2115-2124, 2012.
APA
Fang, Y., Xiang, J., Chen, Z., Gu, X., Li, Z., Tang, F., & Zhou, Z. (2012). miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line. Oncology Reports, 28, 2115-2124. https://doi.org/10.3892/or.2012.2054
MLA
Fang, Y., Xiang, J., Chen, Z., Gu, X., Li, Z., Tang, F., Zhou, Z."miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line". Oncology Reports 28.6 (2012): 2115-2124.
Chicago
Fang, Y., Xiang, J., Chen, Z., Gu, X., Li, Z., Tang, F., Zhou, Z."miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line". Oncology Reports 28, no. 6 (2012): 2115-2124. https://doi.org/10.3892/or.2012.2054
Copy and paste a formatted citation
x
Spandidos Publications style
Fang Y, Xiang J, Chen Z, Gu X, Li Z, Tang F and Zhou Z: miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line. Oncol Rep 28: 2115-2124, 2012.
APA
Fang, Y., Xiang, J., Chen, Z., Gu, X., Li, Z., Tang, F., & Zhou, Z. (2012). miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line. Oncology Reports, 28, 2115-2124. https://doi.org/10.3892/or.2012.2054
MLA
Fang, Y., Xiang, J., Chen, Z., Gu, X., Li, Z., Tang, F., Zhou, Z."miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line". Oncology Reports 28.6 (2012): 2115-2124.
Chicago
Fang, Y., Xiang, J., Chen, Z., Gu, X., Li, Z., Tang, F., Zhou, Z."miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line". Oncology Reports 28, no. 6 (2012): 2115-2124. https://doi.org/10.3892/or.2012.2054
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