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Article

Role of FBXL20 in human colorectal adenocarcinoma

  • Authors:
    • Jianjun Zhu
    • Kai Li
    • Lei Dong
    • Yao Chen
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, Premedical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Institute of Biomedical Engineering, Premedical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 2290-2298
    |
    Published online on: September 27, 2012
       https://doi.org/10.3892/or.2012.2065
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Abstract

In the present study, in order to determine whether the FBXL20 gene plays a role in the carcinogenesis of human colorectal adenocarcinoma, the mRNA expression levels of FBXL20 were examined in 30 pairs of human colorectal adenocarcinoma tissues and corresponding adjacent normal colorectal tissues by quantitative real-time PCR (qRT-PCR). The pGPU/GFP/Neo-FBXL20 siRNA expression vector was transfected into the human colon carcinoma cell lines, SW480 and SW620. The mRNA levels of β-catenin, c-Myc, cyclin D1, p53, PP2A, SET and E-cadherin were determined by qRT-PCR, whereas the protein levels of β-catenin, c-Myc, caspase-3 and SET were examined by western blot analysis. MTT assay and flow cytometry were employed to assess cell proliferation, apoptosis and the cell cycle. As shown by qRT-PCR, FBXL20 mRNA expression was upregulated in 76.7% of the tumor samples. Cell proliferation was inhibited by 33.3% in the SW480-FBXL20 cells and by 22.7% in the SW620-FBXL20 cells, compared to the corresponding control cells. Furthermore, the percentages of apoptotic cells were 21.3 and 17.1% in the SW480-FBXL20 and SW620-FBXL20 cells, whereas the percentages of G1 phase cells in the two cell lines were 90.13 and 78.13%, respectively. In addition, the mRNA expression of SET and E-cadherin was upregulated in the SW480-FBXL20 and SW620-FBXL20 cells, whereas that of β-catenin, c-Myc, cyclin D1, p53 and PP2A was downregulated. Consistently, the protein expression of β-catenin and c-Myc was downregulated, whereas that of SET and caspase-3 was upregulated. Based on these results, we can conclude that FBXL20 plays an essential role in the carcinogenesis of human colorectal adenocarcinoma. Moreover, our data suggest that FBXL20 promotes carcinogenesis through the regulation of the Wnt signaling pathway and caspase activation.
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1 

Bosetti C, Levi F, Rosato V, et al: Recent trends in colorectal cancer mortality in Europe. Int J Cancer. 129:180–191. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Zheng S and Cai SR: Colorectal cancer epidemiology and prevention study in China. Chin Ger J Clin Oncol. 2:72–75. 2003. View Article : Google Scholar

3 

Chen Y, Zhang YZ, Zhou ZG, et al: Identification of differentially expressed genes in human colorectal adenocarcinoma. World J Gastroenterol. 12:1025–1032. 2006.

4 

Zhang C and Chen Y: Electronic cloning and validating of the suppression subtractive hybridization EST ES274070 of human colorectal adenocarcinoma. US Chin J Lymphol Oncol. 6:83–88. 2007.

5 

Zhu JJ, Jiang Q, Yuan LX, et al: Screening and verification of the siRNA targeted to the FBXL20 gene. Chin J Biochem Mol Biol. 27:237–243. 2011.

6 

Skowyra D, Craig KL, Tyers M, et al: F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell. 91:209–219. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Bustin SA, Benes V, Garson JA, et al: The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 55:611–622. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Bai C, Sen PK, Hofman K, et al: SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell. 86:263–274. 1996. View Article : Google Scholar

9 

Craig KL and Tyers M: The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. Prog Biophys Mol Biol. 72:299–328. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Shirane M, Hatakeyama S, Hattori K, et al: Common pathway for the ubiquitination of IkappaBalpha, IkappaBbeta, and IkappaBepsilon mediated by the F-box protein FWD1. J Biol Chem. 274:28169–28174. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Frescas D and Pagano M: Deregulated proteolysis by the F-box proteins SKP2 and β-TrCP: tipping the scales of cancer. Nat Rev Cancer. 8:438–449. 2008.

12 

Kudo Y, Guardavaccaro D, Santamaria PG, et al: Role of F-Box protein beta-Trcp1 in mammary gland development and tumorigenesis. Mol Cell Biol. 24:8184–8194. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Bromberg KD, Kluger HM, Delaunay A, et al: Increased expression of the E3 ubiquitin ligase RNF5 is associated with decreased survival in breast cancer. Cancer Res. 67:8172–8179. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Tsai YC, Mendoza A, Mariano TM, et al: The ubiquitin ligase gp78 promotes sarcoma metastasis by targeting KAl1 for degradation. Nat Med. 13:1504–1509. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Yokoi S, Yasui K, Saito-Ohara F, et al: A novel target gene, SKP2, within the 5p13 amplicon that is frequently detected in small cell lung cancers. Am J Pathol. 161:207–216. 2002. View Article : Google Scholar : PubMed/NCBI

16 

von der Lehr N, Johansson S and Larsson LG: Implication of the ubiquitin/proteasome in Myc-regulated transcription. Cell Cycle. 2:403–407. 2003.PubMed/NCBI

17 

Masuda TA, Inoue H, Sonoda H, et al: Clinical and biological significance of S-phase kinase-associated protein 2 (Skp2) gene expression in gastric carcinoma: modulation of malignant phenotype by Skp2 overexpression, possibly via p27 proteolysis. Cancer Res. 62:3819–3825. 2002.

18 

Gnarra JR and Dressler GR: Expression of Pax-2 in human renal cell carcinoma and growth inhibition by antisense oligonucleotides. Cancer Res. 55:4092–4098. 1995.PubMed/NCBI

19 

Kim M, Yan Y, Lee K, et al: Ectopic expression of Von Hippel-Lindau tumor suppressor induces apoptosis in 786-O renal cell carcinoma cells and regresses tumor growth of 786-O cells in nude mouse. Biochemical Biophys Res Commun. 320:945–950. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Maxwell PH, Wiesener MS, Chanq GW, et al: The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis. Nature. 399:271–275. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Segditsas S and Tomlinson I: Colorectal cancer and genetic alterations in the Wnt pathway. Oncogene. 25:7531–7537. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Jung P, Menssen A, Mayr D and Hermeking H: AP4 encodes a c-MYC-inducible repressor of p21. Proc Natl Acad Sci USA. 105:15046–15051. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Chu IM, Hengst L and Slingerland JM: The Cdk inhibitor p27 in human cancer: prognostic potential and relevance to anticancer therapy. Nat Rev Cancer. 8:253–267. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Koldobskiy MA, Chakraborty A, Werner JK Jr, et al: p53-mediated apoptosis requires inositol hexakisphosphate kinase-2. Proc Natl Acad Sci USA. 107:20947–20951. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Kim Y, Reifenberger G, Lu D, et al: Influencing the Wnt signaling pathway in multiple myeloma. Anticancer Res. 31:725–730. 2011.PubMed/NCBI

26 

Tian X, Liu Z, Niu B, et al: E-cadherin/β-catenin complex and the epithelial barrier. J Biomed Biotechnol. 2011:5673052011.

27 

Jiang Q, Zhang C, Zhu J, et al: The set gene is a potential oncogene in human colorectal adenocarcinoma and oral squamous cell carcinoma. Mol Med Rep. 4:993–999. 2011.PubMed/NCBI

28 

Willert K, Shibamoto S and Nusse R: Wnt-induced dephosphorylation of axin releases beta-catenin from the axin complex. Genes Dev. 13:1768–1773. 1999. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Li K, Dong L and Chen Y: Role of FBXL20 in human colorectal adenocarcinoma. Oncol Rep 28: 2290-2298, 2012.
APA
Zhu, J., Li, K., Dong, L., & Chen, Y. (2012). Role of FBXL20 in human colorectal adenocarcinoma. Oncology Reports, 28, 2290-2298. https://doi.org/10.3892/or.2012.2065
MLA
Zhu, J., Li, K., Dong, L., Chen, Y."Role of FBXL20 in human colorectal adenocarcinoma". Oncology Reports 28.6 (2012): 2290-2298.
Chicago
Zhu, J., Li, K., Dong, L., Chen, Y."Role of FBXL20 in human colorectal adenocarcinoma". Oncology Reports 28, no. 6 (2012): 2290-2298. https://doi.org/10.3892/or.2012.2065
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Li K, Dong L and Chen Y: Role of FBXL20 in human colorectal adenocarcinoma. Oncol Rep 28: 2290-2298, 2012.
APA
Zhu, J., Li, K., Dong, L., & Chen, Y. (2012). Role of FBXL20 in human colorectal adenocarcinoma. Oncology Reports, 28, 2290-2298. https://doi.org/10.3892/or.2012.2065
MLA
Zhu, J., Li, K., Dong, L., Chen, Y."Role of FBXL20 in human colorectal adenocarcinoma". Oncology Reports 28.6 (2012): 2290-2298.
Chicago
Zhu, J., Li, K., Dong, L., Chen, Y."Role of FBXL20 in human colorectal adenocarcinoma". Oncology Reports 28, no. 6 (2012): 2290-2298. https://doi.org/10.3892/or.2012.2065
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