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Article Open Access

Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor

  • Authors:
    • Kosei Nakajima
    • Takashi Yanagawa
    • Hideomi Watanabe
    • Kenji Takagishi
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan, Department of Physical Therapy, Graduate School of Health Science, Gunma University, Maebashi, Gunma, Japan
    Copyright: © Nakajima et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1953-1958
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    Published online on: October 1, 2012
       https://doi.org/10.3892/or.2012.2066
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Abstract

Autocrine motility factor (AMF) plays an important role in the development of metastasis by regulating tumor cell motility. The expression of AMF is associated with metastasis in malignant musculoskeletal tumors including osteosarcoma. Recent studies indicated that hyperthermia contributes to the improvement of the prognosis of patients with soft tissue sarcomas; however, few reports have evaluated the impact of hyperthermia on tumor cell motility, which is an important factor of metastasis. The purpose of this study was to evaluate the effect of hyperthermia with or without heat shock protein (HSP) inhibitors on the motility and AMF expression in an osteosarcoma cell line. Hyperthermia was carried out at 41˚C for 24 h. According to microarray results, HSP90, HSP70 and HSP27 expression was upregulated in osteosarcoma cells under hyperthermia. The intracellular, secreted AMF, mRNA of AMF and cell motility were evaluated by western blotting, ELISA, RT-PCR, wound healing and phagokinetic track assays, respectively. The protein secretion and mRNA levels of AMF and tumor cell motility were significantly decreased by hyperthermia. Of note, the downregulated AMF expression and motility were recovered by the addition of an HSP27 inhibitor. By contrast, the HSP90 and HSP70/72/105 inhibitors had no effect on AMF expression and motility downregulated by hyperthermia. In conclusion, hyperthermia reduced AMF expression and tumor cell motility via HSP27 and may therefore be applied as osteosarcoma treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Nakajima K, Yanagawa T, Watanabe H and Takagishi K: Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor. Oncol Rep 28: 1953-1958, 2012.
APA
Nakajima, K., Yanagawa, T., Watanabe, H., & Takagishi, K. (2012). Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor. Oncology Reports, 28, 1953-1958. https://doi.org/10.3892/or.2012.2066
MLA
Nakajima, K., Yanagawa, T., Watanabe, H., Takagishi, K."Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor". Oncology Reports 28.6 (2012): 1953-1958.
Chicago
Nakajima, K., Yanagawa, T., Watanabe, H., Takagishi, K."Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor". Oncology Reports 28, no. 6 (2012): 1953-1958. https://doi.org/10.3892/or.2012.2066
Copy and paste a formatted citation
x
Spandidos Publications style
Nakajima K, Yanagawa T, Watanabe H and Takagishi K: Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor. Oncol Rep 28: 1953-1958, 2012.
APA
Nakajima, K., Yanagawa, T., Watanabe, H., & Takagishi, K. (2012). Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor. Oncology Reports, 28, 1953-1958. https://doi.org/10.3892/or.2012.2066
MLA
Nakajima, K., Yanagawa, T., Watanabe, H., Takagishi, K."Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor". Oncology Reports 28.6 (2012): 1953-1958.
Chicago
Nakajima, K., Yanagawa, T., Watanabe, H., Takagishi, K."Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor". Oncology Reports 28, no. 6 (2012): 1953-1958. https://doi.org/10.3892/or.2012.2066
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