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Article

Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β1

  • Authors:
    • Hekun Jin
    • Xiaoxue Xie
    • Bingqiang Hu
    • Fuping Gao
    • Jumei Zhou
    • Yingying Zhang
    • Lehui Du
    • Xiaowen Wang
    • Lingyun Zhao
    • Xiaodong Zhang
    • Liangfang Shen
    • Yuping Liao
    • Jintian Tang
  • View Affiliations / Copyright

    Affiliations: Xiangya Hospital of Central South University, Changsha 410008, P.R. China, Affiliated Tumor Hospital of Xiangya Medical School, Central South University, Changsha 410013, P.R. China, Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, P.R. China, Key Laboratory of Particle and Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, P.R. China
  • Pages: 725-734
    |
    Published online on: November 7, 2012
       https://doi.org/10.3892/or.2012.2128
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Abstract

The degradation of basement membranes by tumor cells involves secretion and activation of proteinases, such as the matrix metalloproteinases (MMPs), and results from an imbalance between their inhibitors and activators that are controlled by various growth factors or cytokines, among which TGF-β1 may be the most intriguing. In order to study the therapeutic effect and molecular mechanism of hyperthermia on aggressive malignant melanoma, the expression levels of TGF-β1 and Smad4 in B16F10 cells were dynamically analyzed by RT-PCR and western blotting for 24 h after heat treatment, from which time-dependent changes were determined. As expected, the proliferation and invasive ability of B16F10 cells were suppressed strongly by heat treatment. Furthermore, we compared the expression of TGF-β1 in melanoma mouse models before and after magnetic fluid hyperthermia (MFH) in vivo. After hyperthermia, the tumor growth rate was reduced with a decline in TGF-β1 protein expression. We conclude that changes in the TGF-β1 pathway induced by hyperthermia may be an important part of the molecular mechanism involved.
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Copy and paste a formatted citation
Spandidos Publications style
Jin H, Xie X, Hu B, Gao F, Zhou J, Zhang Y, Du L, Wang X, Zhao L, Zhang X, Zhang X, et al: Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β1. Oncol Rep 29: 725-734, 2013.
APA
Jin, H., Xie, X., Hu, B., Gao, F., Zhou, J., Zhang, Y. ... Tang, J. (2013). Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β1. Oncology Reports, 29, 725-734. https://doi.org/10.3892/or.2012.2128
MLA
Jin, H., Xie, X., Hu, B., Gao, F., Zhou, J., Zhang, Y., Du, L., Wang, X., Zhao, L., Zhang, X., Shen, L., Liao, Y., Tang, J."Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β1". Oncology Reports 29.2 (2013): 725-734.
Chicago
Jin, H., Xie, X., Hu, B., Gao, F., Zhou, J., Zhang, Y., Du, L., Wang, X., Zhao, L., Zhang, X., Shen, L., Liao, Y., Tang, J."Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β1". Oncology Reports 29, no. 2 (2013): 725-734. https://doi.org/10.3892/or.2012.2128
Copy and paste a formatted citation
x
Spandidos Publications style
Jin H, Xie X, Hu B, Gao F, Zhou J, Zhang Y, Du L, Wang X, Zhao L, Zhang X, Zhang X, et al: Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β1. Oncol Rep 29: 725-734, 2013.
APA
Jin, H., Xie, X., Hu, B., Gao, F., Zhou, J., Zhang, Y. ... Tang, J. (2013). Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β1. Oncology Reports, 29, 725-734. https://doi.org/10.3892/or.2012.2128
MLA
Jin, H., Xie, X., Hu, B., Gao, F., Zhou, J., Zhang, Y., Du, L., Wang, X., Zhao, L., Zhang, X., Shen, L., Liao, Y., Tang, J."Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β1". Oncology Reports 29.2 (2013): 725-734.
Chicago
Jin, H., Xie, X., Hu, B., Gao, F., Zhou, J., Zhang, Y., Du, L., Wang, X., Zhao, L., Zhang, X., Shen, L., Liao, Y., Tang, J."Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β1". Oncology Reports 29, no. 2 (2013): 725-734. https://doi.org/10.3892/or.2012.2128
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