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Article

The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages

  • Authors:
    • Zhanlong Shen
    • Yingjiang Ye
    • Tuuli Kauttu
    • Hanna Seppänen
    • Sanna  Vainionpää
    • Shan Wang
    • Harri Mustonen
    • Pauli Puolakkainen
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, P.R. China, Department of Surgery, Helsinki University Central Hospital, and University of Helsinki, Helsinki 00290, Finland
  • Pages: 791-797
    |
    Published online on: November 14, 2012
       https://doi.org/10.3892/or.2012.2137
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Abstract

Kindlin-2 is a novel focal adhesion gene mediating the cell-extracellular matrix (ECM) adhesion. Tumor-associated macrophages (TAMs) play an important role in linking chronic inflammation to cancer progression. Both kindlin-2 and TAMs have been found to promote the invasion of gastric cancer cells in our previous studies. However, the correlation between kindlin-2 and TAMs remains unclear. Real-time RT-PCR was used to investigate kindlin-2 expression in the AGS, NCI and Hs-746T gastric cancer cell lines co-cultured with TAMs under normal or hypoxic conditions. IL8, IL10, IL11, IL17b, IL18, IL22 and IL24 expressions were measured by real-time RT-PCR in the gastric cancer lines with varying levels of kindlin-2 expression, as well as after downregulation of kindlin-2 mRNA expression by the siRNA method. We found that kindlin-2 was upregulated in all three gastric cancer cell lines when co-cultured with TAMs under normal conditions. Under hypoxic conditions, the induction of kindlin-2 expression induced by macrophages was significantly downregulated in the Hs-746T cell line. IL8, IL11, IL17b, IL22 and IL24 expression was significantly higher in gastric cell lines with high kindlin-2 expression. Downregulation of kindlin-2 mRNA decreased IL10, IL11, IL17b, IL22 and IL24 expression but IL8 and IL18 expression was upregulated. Therefore, the novel focal adhesion gene kindlin-2 may play an important role in promoting the invasion of gastric cancer cells mediated by TAMs through regulating interleukin expression.
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Copy and paste a formatted citation
Spandidos Publications style
Shen Z, Ye Y, Kauttu T, Seppänen H, Vainionpää S, Wang S, Mustonen H and Puolakkainen P: The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages. Oncol Rep 29: 791-797, 2013.
APA
Shen, Z., Ye, Y., Kauttu, T., Seppänen, H., Vainionpää, S., Wang, S. ... Puolakkainen, P. (2013). The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages. Oncology Reports, 29, 791-797. https://doi.org/10.3892/or.2012.2137
MLA
Shen, Z., Ye, Y., Kauttu, T., Seppänen, H., Vainionpää, S., Wang, S., Mustonen, H., Puolakkainen, P."The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages". Oncology Reports 29.2 (2013): 791-797.
Chicago
Shen, Z., Ye, Y., Kauttu, T., Seppänen, H., Vainionpää, S., Wang, S., Mustonen, H., Puolakkainen, P."The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages". Oncology Reports 29, no. 2 (2013): 791-797. https://doi.org/10.3892/or.2012.2137
Copy and paste a formatted citation
x
Spandidos Publications style
Shen Z, Ye Y, Kauttu T, Seppänen H, Vainionpää S, Wang S, Mustonen H and Puolakkainen P: The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages. Oncol Rep 29: 791-797, 2013.
APA
Shen, Z., Ye, Y., Kauttu, T., Seppänen, H., Vainionpää, S., Wang, S. ... Puolakkainen, P. (2013). The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages. Oncology Reports, 29, 791-797. https://doi.org/10.3892/or.2012.2137
MLA
Shen, Z., Ye, Y., Kauttu, T., Seppänen, H., Vainionpää, S., Wang, S., Mustonen, H., Puolakkainen, P."The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages". Oncology Reports 29.2 (2013): 791-797.
Chicago
Shen, Z., Ye, Y., Kauttu, T., Seppänen, H., Vainionpää, S., Wang, S., Mustonen, H., Puolakkainen, P."The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages". Oncology Reports 29, no. 2 (2013): 791-797. https://doi.org/10.3892/or.2012.2137
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