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Article

Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells

  • Authors:
    • Dong Xu
    • Xingxing He
    • Ying Chang
    • Chuanrui Xu
    • Xiang Jiang
    • Shuzhen Sun
    • Jusheng Lin
  • View Affiliations / Copyright

    Affiliations: Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
  • Pages: 653-661
    |
    Published online on: November 14, 2012
       https://doi.org/10.3892/or.2012.2138
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Abstract

microRNAs (miRNAs) are negative regulators of gene expression and can function as tumor suppressors or oncogenes. Several miRNAs are associated with the development of hepatocellular carcinoma (HCC). miR-96 has been closely associated with cell proliferation and clonogenicity. Upregulation of miR-96 has been observed in various types of cancer. However, the biological function of miR-96 in hepatocarcinogenesis remains largely unknown. In this study, we demonstrated that miR-96 was upregulated in HCC and inhibition of miR-96 significantly suppressed HCC cell proliferation and colony formation. The expression levels of forkhead box O1 (FOXO1) and forkhead box O3a (FOXO3a) were upregulated when miR-96 was inhibited in HCC cells and the inhibition of FOXO1 and FOXO3a promoted HCC cell proliferation and colony formation. Collectively, these data reveal an important contribution of miR-96 to hepatocarcinogenesis and suggest a role for FOXO1 and FOXO3a dysregulation in this process. Thus, the use of a synthetic inhibitor of miR-96 may be a promising approach for the treatment of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Xu D, He X, Chang Y, Xu C, Jiang X, Sun S and Lin J: Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells. Oncol Rep 29: 653-661, 2013.
APA
Xu, D., He, X., Chang, Y., Xu, C., Jiang, X., Sun, S., & Lin, J. (2013). Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells. Oncology Reports, 29, 653-661. https://doi.org/10.3892/or.2012.2138
MLA
Xu, D., He, X., Chang, Y., Xu, C., Jiang, X., Sun, S., Lin, J."Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells". Oncology Reports 29.2 (2013): 653-661.
Chicago
Xu, D., He, X., Chang, Y., Xu, C., Jiang, X., Sun, S., Lin, J."Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells". Oncology Reports 29, no. 2 (2013): 653-661. https://doi.org/10.3892/or.2012.2138
Copy and paste a formatted citation
x
Spandidos Publications style
Xu D, He X, Chang Y, Xu C, Jiang X, Sun S and Lin J: Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells. Oncol Rep 29: 653-661, 2013.
APA
Xu, D., He, X., Chang, Y., Xu, C., Jiang, X., Sun, S., & Lin, J. (2013). Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells. Oncology Reports, 29, 653-661. https://doi.org/10.3892/or.2012.2138
MLA
Xu, D., He, X., Chang, Y., Xu, C., Jiang, X., Sun, S., Lin, J."Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells". Oncology Reports 29.2 (2013): 653-661.
Chicago
Xu, D., He, X., Chang, Y., Xu, C., Jiang, X., Sun, S., Lin, J."Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells". Oncology Reports 29, no. 2 (2013): 653-661. https://doi.org/10.3892/or.2012.2138
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