A new in vivo model to analyze hepatic metastasis of the human colon cancer cell line HCT116 in NOD/Shi-scid/IL-2Rγnull (NOG) mice by 18F-FDG PET/CT

  • Authors:
    • Kenji Kawai
    • Katsumi Tamura
    • Ikuko Sakata
    • Jiro Ishida
    • Masayoshi Nagata
    • Hideo Tsukada
    • Hiroshi Suemizu
    • Masato Nakamura
    • Yoshiyuki Abe
    • Tsuyoshi Chijiwa
  • View Affiliations

  • Published online on: November 15, 2012     https://doi.org/10.3892/or.2012.2141
  • Pages: 464-468
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Abstract

Clinically, 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG-PET/CT) is useful in the evaluation of various types of human cancers. While PET analysis has been established to evaluate subcutaneous lesions of human cancers in mice, its applications for internal lesions are still being developed. We are currently evaluating new PET approaches for the effective evaluation of in vivo metastatic lesions in the internal organs of small experimental animals. In this study, we analyzed in vivo hepatic metastases of human colonic cancer in immunodeficient mice (NOD/Shi-scid/IL-2Rγnull, NOG) using PET imaging. This new PET approach has been proposed for the evaluation of in vivo metastatic lesions in internal organs. The human colon cancer line HCT116 (1.0x105 and 1.0x106 cells/mouse) was transplanted by intrasplenic injection. 18F-FDG-PET/CT scans were performed 2 weeks after transplantation. After PET/CT scans, histopathological examinations were performed. PET/CT analysis disclosed multiple metastatic foci and increased standardized uptake values (SUV) of FDG in the livers of NOG mice (control, SUVmean 0.450±0.033, SUVmax 0.635±0.017; 1.0x105 cells, 0.853±0.087, 1.254±0.237; 1.0x106 cells, 1.211±0.108, 1.701±0.158). There were significant differences in FDG uptakes between the three groups (ANOVA, P=0.017 in SUVmean; P=0.044 in SUVmax, n=2). We clearly and quantitatively detected images of hepatic metastasis in the livers of NOG mice by 18F-FDG-PET/CT in vivo. PET/CT analysis of internal organ lesions of human cancerous xenografts is a new reliable experimental system to simulate metastases. This model system is useful for analyzing metastatic mechanisms and for developing new novel drugs targeting hepatic metastases of cancer.
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February 2013
Volume 29 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kawai K, Tamura K, Sakata I, Ishida J, Nagata M, Tsukada H, Suemizu H, Nakamura M, Abe Y, Chijiwa T, Chijiwa T, et al: A new in vivo model to analyze hepatic metastasis of the human colon cancer cell line HCT116 in NOD/Shi-scid/IL-2Rγnull (NOG) mice by 18F-FDG PET/CT. Oncol Rep 29: 464-468, 2013
APA
Kawai, K., Tamura, K., Sakata, I., Ishida, J., Nagata, M., Tsukada, H. ... Chijiwa, T. (2013). A new in vivo model to analyze hepatic metastasis of the human colon cancer cell line HCT116 in NOD/Shi-scid/IL-2Rγnull (NOG) mice by 18F-FDG PET/CT. Oncology Reports, 29, 464-468. https://doi.org/10.3892/or.2012.2141
MLA
Kawai, K., Tamura, K., Sakata, I., Ishida, J., Nagata, M., Tsukada, H., Suemizu, H., Nakamura, M., Abe, Y., Chijiwa, T."A new in vivo model to analyze hepatic metastasis of the human colon cancer cell line HCT116 in NOD/Shi-scid/IL-2Rγnull (NOG) mice by 18F-FDG PET/CT". Oncology Reports 29.2 (2013): 464-468.
Chicago
Kawai, K., Tamura, K., Sakata, I., Ishida, J., Nagata, M., Tsukada, H., Suemizu, H., Nakamura, M., Abe, Y., Chijiwa, T."A new in vivo model to analyze hepatic metastasis of the human colon cancer cell line HCT116 in NOD/Shi-scid/IL-2Rγnull (NOG) mice by 18F-FDG PET/CT". Oncology Reports 29, no. 2 (2013): 464-468. https://doi.org/10.3892/or.2012.2141