Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
February 2013 Volume 29 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February 2013 Volume 29 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses

  • Authors:
    • Hongyan Wu
    • Yu Han
    • Ye Qin
    • Chunyu Cao
    • Yan Xia
    • Changbai Liu
    • Yanlin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China, Institute of Molecular Biology, Three Gorges University Medical College, Yichang, Hubei 443002, P.R. China
  • Pages: 529-534
    |
    Published online on: November 15, 2012
       https://doi.org/10.3892/or.2012.2142
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

It has been reported that calreticulin (CRT) plays an important role in mediating immunogenic tumour cell death. In the process of tumour cell apoptosis induced by specific stimuli, CRT is quickly transferred from the endoplasmic reticulum to the cell membrane. As a specific ligand, CRT on the surface of apoptotic tumour cells could mediate the recognition and clearance of apoptotic tumour cells by professional and non-professional phagocytes. In our previous studies, we used B16-F1 mouse melanoma cells coated with mCRT-vGPCR (a recombinant fusion protein of mouse CRT and virus G-protein-coupled receptor) as a whole-cell tumour vaccine to immunise experimental animals and found that this whole-cell vaccine could strongly inhibit the growth of homologous tumours. In this study, we further evaluated immune responses induced by this mCRT-vGPCR-coated whole-cell vaccine both in vivo and in vitro. An in vitro phagocytosis assay showed that the mCRT-vGPCR on the cell surface greatly enhanced the engulfment of B16-F1 cells by dendritic cells (DCs). The specific antitumour immune response was observed when the mCRT-vGPCR-coated B16-F1 cells were used as a whole-cell tumour vaccine to immune mice, which included significantly enhanced cytotoxic T lymphocyte (CTL) activities and increased the number of IFN-γ-producing T cells. These results indicate that the mCRT-vGPCR-coated whole-cell vaccine can induce specific antitumour immunity though the activation of DCs. These results may provide an experimental basis for the development of new tumour vaccines.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Zitvogel L, Tesniere A and Kroemer G: Cancer despite immunosurveillance: immunoselection and immunosubversion. Nat Rev Immunol. 6:715–727. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Huang X, Ye D and Thorpe PE: Enhancing the potency of a whole-cell breast cancer vaccine in mice with an antibody-IL-2 immunocytokine that targets exposed phosphatidylserine. Vaccine. 29:4785–4793. 2011. View Article : Google Scholar

3 

Johnson S, Michalak M, Opas M and Eggleton P: The ins and outs of calreticulin: from the ER lumen to the extracellular space. Trends Cell Biol. 11:122–129. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Michalak M, Corbett EF, Mesaeli N, et al: Calreticulin: one protein, one gene, many functions. Biochem J. 344:281–292. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Trombetta ES and Parodi AJ: Quality control and protein folding in the secretory pathway. Annu Rev Cell Dev Biol. 19:649–676. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Trombetta ES: The contribution of N-glycans and their processing in the endoplasmic reticulum to glycoprotein biosynthesis. Glycobiology. 13:R77–R91. 2003.

7 

Michalak M, Robert Parker JM and Opas M: Ca2+ signaling and calcium binding chaperones of the endoplasmic reticulum. Cell Calcium. 32:269–278. 2002.

8 

Zhu Q, Zelinka P, White T and Tanzer ML: Calreticulin-integrin bidirectional signaling complex. Biochem Biophys Res Commun. 232:354–358. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Kwon MS, Park CS, Choi K, et al: Calreticulin couples calcium release and calcium influx in integrin-mediated calcium signaling. Mol Biol Cell. 11:1433–1443. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Gray AJ, Park PW, Broekelmann TJ, et al: The mitogenic effects of the B-chain of fibrinogen are mediated through cell surface calreticulin. J Biol Chem. 270:26602–26606. 1995. View Article : Google Scholar : PubMed/NCBI

11 

White TK, Zhu Q and Tanzer ML: Cell surface calreticulin is a putative mannoside lectin which triggers mouse melanoma cell spreading. J Biol Chem. 270:15926–15929. 1995. View Article : Google Scholar : PubMed/NCBI

12 

Obeid M, Tesniere A, Ghiringhelli F, et al: Calreticulin exposure dictates the immunogenicity of cancer cell death. Nat Med. 13:54–61. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Obeid M, Panaretakis T, Joza N, et al: Calreticulin exposure is required for the immunogenicity of gamma irradiation and UVC light-induced apoptosis. Cell Death Differ. 14:1848–1850. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Spisek R and Dhodapkar MV: Towards a better way to die with chemotherapy: role of heat shock protein exposure on dying tumor cells. Cell Cycle. 6:1962–1965. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Spisek R, Charalambous A, Mazumder A, Vesole DH, Jagannath S and Dhodapkar MV: Bortezomib enhances dendritic cell (DC)-mediated induction of immunity to human myeloma via exposure of cell surface heat shock protein 90 on dying tumor cells: therapeutic implications. Blood. 109:4839–4845. 2007. View Article : Google Scholar

16 

Tesniere A, Panaretakis T, Kepp O, et al: Molecular characteristics of immunogenic cancer cell death. Cell Death Differ. 15:3–12. 2008. View Article : Google Scholar

17 

Panaretakis T, Joza N, Modjtahedi N, et al: The co-translocation of ERp57 and calreticulin determines the immunogenicity of cell death. Cell Death Differ. 15:1499–1509. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Qin Y, Han Y, Cao C, Ren Y, Li C and Wang Y: Melanoma B16-F1 cells coated with fusion protein of mouse calreticulin and virus G-protein coupled receptor induced the antitumor immune response in Balb/C mice. Cancer Biol Ther. 11:574–580. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Copier J and Dalgleish A: Overview of tumor cell-based vaccines. Int Rev Immunol. 25:297–319. 2006. View Article : Google Scholar

20 

de Gruijl TD, van den Eertwegh AJ, Pinedo HM and Scheper RJ: Whole-cell cancer vaccination: from autologous to allogeneic tumor- and dendritic cell-based vaccines. Cancer Immunol Immunother. 57:1569–1577. 2008.PubMed/NCBI

21 

Chiang CL, Benencia F and Coukos G: Whole tumor antigen vaccines. Semin Immunol. 22:132–143. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Copier J and Dalgleish A: Whole-cell vaccines: a failure or a success waiting to happen? Curr Opin Mol Ther. 12:14–20. 2010.PubMed/NCBI

23 

Bodey B, Bodey B Jr, Siegel SE and Kaiser HE: Failure of cancer vaccines: the significant limitations of this approach to immunotherapy. Anticancer Res. 20:2665–2676. 2000.PubMed/NCBI

24 

Cohen EP, Chopra A, O-Sullivan I and Kim TS: Enhancing cellular cancer vaccines. Immunotherapy. 1:495–504. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Fucikova J, Kralikova P, Fialova A, et al: Human tumor cells killed by anthracyclines induce a tumor-specific immune response. Cancer Res. 71:4821–4833. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Liu C, Sandford G, Fei G and Nicholas J: G protein selectivity determinant specified by a viral chemokine receptor-conserved region in the C tail of the human herpes virus 8 G protein-coupled receptor. J Virol. 78:2460–2471. 2004. View Article : Google Scholar

27 

Cao CY, Han Y, Ren YS and Wang YL: Apoptotic B16-F1 cells coated with recombinant calreticulin mediated anti-tumor immune response in mice. Chin J Cancer Res. 22:253–259. 2010. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wu H, Han Y, Qin Y, Cao C, Xia Y, Liu C and Wang Y: Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses. Oncol Rep 29: 529-534, 2013.
APA
Wu, H., Han, Y., Qin, Y., Cao, C., Xia, Y., Liu, C., & Wang, Y. (2013). Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses. Oncology Reports, 29, 529-534. https://doi.org/10.3892/or.2012.2142
MLA
Wu, H., Han, Y., Qin, Y., Cao, C., Xia, Y., Liu, C., Wang, Y."Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses". Oncology Reports 29.2 (2013): 529-534.
Chicago
Wu, H., Han, Y., Qin, Y., Cao, C., Xia, Y., Liu, C., Wang, Y."Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses". Oncology Reports 29, no. 2 (2013): 529-534. https://doi.org/10.3892/or.2012.2142
Copy and paste a formatted citation
x
Spandidos Publications style
Wu H, Han Y, Qin Y, Cao C, Xia Y, Liu C and Wang Y: Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses. Oncol Rep 29: 529-534, 2013.
APA
Wu, H., Han, Y., Qin, Y., Cao, C., Xia, Y., Liu, C., & Wang, Y. (2013). Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses. Oncology Reports, 29, 529-534. https://doi.org/10.3892/or.2012.2142
MLA
Wu, H., Han, Y., Qin, Y., Cao, C., Xia, Y., Liu, C., Wang, Y."Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses". Oncology Reports 29.2 (2013): 529-534.
Chicago
Wu, H., Han, Y., Qin, Y., Cao, C., Xia, Y., Liu, C., Wang, Y."Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses". Oncology Reports 29, no. 2 (2013): 529-534. https://doi.org/10.3892/or.2012.2142
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team