SLC gene-modified dendritic cells mediate T cell-dependent anti-gastric cancer immune responses in vitro

  • Authors:
    • Gang Xue
    • Ying Cheng
    • Feng Ran
    • Xianhui Li
    • Tao Huang
    • Yong Yang
    • Yanbiao Zhang
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  • Published online on: November 28, 2012     https://doi.org/10.3892/or.2012.2154
  • Pages: 595-604
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Abstract

Dendritic cells (DCs) are potent professional antigen-presenting cells (APCs) with the ability to prime naïve T cells, and play an important role in the initiation and regulation of immune responses. In this study, we constructed a recombinant adenovirus carrying the SLC gene (Ad-SLC), and detected the biological effects of Ad-SLC-modified DCs as an adjuvant for the initiation of gastric cancer immune responses. Human DCs were transfected with Ad-SLC and the recombinant adenovirus carrying the β-galactosidase gene, Ad-LacZ, respectively. Modified DCs were pulsed with the cell lysate antigen of SGC-7901 cells (a type of gastric cancer cell line) and co-cultured with autologous T cells. The T cells were harvested and incubated with SGC-7901 cells and the cytotoxic function of the T cells was detected. Based on the data, the expression of mature DC phenotypes CD83 and CCR7 was upregulated after transfection with Ad-SLC and the chemotaxis function of DCs was augmented after transfection with Ad-SLC. Moreover, the expression of RANTES in DCs was upregul­ated by Ad-SLC transfection, while expression levels of IL-12p70 and IL-10 were not significantly altered. When co-cultured with autologous T cells, DCs modified with the SLC gene and pulsed with SGC-7901 cell lysates significantly promoted the proliferation of autologous T cells and induced Th1 differenti­ation, and displayed a strong cytotoxicity to SGC-7901 cells. In conclusion, Ad-SLC promoted DC maturation, enhancing the ability of DCs for T-cell chemotaxis and T-cell stimulation, and induced specific anti-gastric cancer cellular immunity. Recombinant Ad-SLC-modified DCs may be used as an adjuvant to induce an effective anti-gastric cancer immune response.
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February 2013
Volume 29 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xue G, Cheng Y, Ran F, Li X, Huang T, Yang Y and Zhang Y: SLC gene-modified dendritic cells mediate T cell-dependent anti-gastric cancer immune responses in vitro. Oncol Rep 29: 595-604, 2013
APA
Xue, G., Cheng, Y., Ran, F., Li, X., Huang, T., Yang, Y., & Zhang, Y. (2013). SLC gene-modified dendritic cells mediate T cell-dependent anti-gastric cancer immune responses in vitro. Oncology Reports, 29, 595-604. https://doi.org/10.3892/or.2012.2154
MLA
Xue, G., Cheng, Y., Ran, F., Li, X., Huang, T., Yang, Y., Zhang, Y."SLC gene-modified dendritic cells mediate T cell-dependent anti-gastric cancer immune responses in vitro". Oncology Reports 29.2 (2013): 595-604.
Chicago
Xue, G., Cheng, Y., Ran, F., Li, X., Huang, T., Yang, Y., Zhang, Y."SLC gene-modified dendritic cells mediate T cell-dependent anti-gastric cancer immune responses in vitro". Oncology Reports 29, no. 2 (2013): 595-604. https://doi.org/10.3892/or.2012.2154