Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma

Yoshizawa,Kunio; Nozaki,Shinichi; Kato,Aki; Hirai,Mariko; Yanase,Mizuki; Yoshimoto,Taisuke; Kimura,Iyo; Sugiura,Shiro; Okamune,Ayako; Kitahara,Hiroko; Noguchi,Natsuyo; Kato,Koroku; Ueki,Koichiro; Kawashiri,Shuichi

doi:10.3892/or.2012.2161

Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma

Authors:
- Kunio Yoshizawa
- Shinichi Nozaki
- Aki Kato
- Mariko Hirai
- Mizuki Yanase
- Taisuke Yoshimoto
- Iyo Kimura
- Shiro Sugiura
- Ayako Okamune
- Hiroko Kitahara
- Natsuyo Noguchi
- Koroku Kato
- Koichiro Ueki
- Shuichi Kawashiri
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Affiliations: Department of Oral and Maxillofacial Surgery, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa 920-8641, Japan, Department of Dentistry and Oral Surgery, National Hospital Organization, Kanazawa Medical Center, Kanazawa, Ishikawa 920-8650, Japan
Published online on: November 30, 2012 https://doi.org/10.3892/or.2012.2161
Pages: 445-450

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Abstract

Claudin-7 belongs to the claudin family, which consists of 24 subtypes of essential tight junction (TJ) integral membrane proteins with molecular weights of 20-27 kDa. We investigated the interrelationship between clinicopathological findings and claudin-7 expression in oral squamous cell carcinoma (OSCC). Using immunohistochemical techniques to examine the expression levels of claudin-7 in 67 cases of OSCC, claudin-7 expression was detected in 35 (52.2%) of the 67 cases. We also compared the clinicopathological features of the OSCC cases with claudin-7 expression levels. Moreover, six cell lines with various invasive properties were investigated in vitro to compare mRNA and protein levels of claudin-7 using reverse transcription-polymerase chain reaction (RT-PCR) and the western blotting method. Decreased claudin-7 expression correlated significantly with T-category (p<0.05), lymph node metastasis (p<0.01), and mode of invasion (p<0.001). Patients with positive claudin-7 expression had a significantly better prognosis (p<0.05). Claudin-7 protein and mRNA levels were lower in the HOC313 and TSU cells, which have higher invasive potentials compared with other cell lines. These results suggest that loss of claudin-7 expression is associated closely with invasion and lymph metastasis and is an unfavorable prognostic factor in patients with OSCC.

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