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Article

Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma

  • Authors:
    • Kunio Yoshizawa
    • Shinichi Nozaki
    • Aki Kato
    • Mariko Hirai
    • Mizuki Yanase
    • Taisuke Yoshimoto
    • Iyo Kimura
    • Shiro Sugiura
    • Ayako Okamune
    • Hiroko Kitahara
    • Natsuyo Noguchi
    • Koroku Kato
    • Koichiro Ueki
    • Shuichi Kawashiri
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa 920-8641, Japan, Department of Dentistry and Oral Surgery, National Hospital Organization, Kanazawa Medical Center, Kanazawa, Ishikawa 920-8650, Japan
  • Pages: 445-450
    |
    Published online on: November 30, 2012
       https://doi.org/10.3892/or.2012.2161
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Abstract

Claudin-7 belongs to the claudin family, which consists of 24 subtypes of essential tight junction (TJ) integral membrane proteins with molecular weights of 20-27 kDa. We investigated the interrelationship between clinicopathological findings and claudin-7 expression in oral squamous cell carcinoma (OSCC). Using immunohistochemical techniques to examine the expression levels of claudin-7 in 67 cases of OSCC, claudin-7 expression was detected in 35 (52.2%) of the 67 cases. We also compared the clinicopathological features of the OSCC cases with claudin-7 expression levels. Moreover, six cell lines with various invasive properties were investigated in vitro to compare mRNA and protein levels of claudin-7 using reverse transcription-polymerase chain reaction (RT-PCR) and the western blotting method. Decreased claudin-7 expression correlated significantly with T-category (p<0.05), lymph node metastasis (p<0.01), and mode of invasion (p<0.001). Patients with positive claudin-7 expression had a significantly better prognosis (p<0.05). Claudin-7 protein and mRNA levels were lower in the HOC313 and TSU cells, which have higher invasive potentials compared with other cell lines. These results suggest that loss of claudin-7 expression is asso­ciated closely with invasion and lymph metastasis and is an unfavorable prognostic factor in patients with OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yoshizawa K, Nozaki S, Kato A, Hirai M, Yanase M, Yoshimoto T, Kimura I, Sugiura S, Okamune A, Kitahara H, Kitahara H, et al: Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma. Oncol Rep 29: 445-450, 2013.
APA
Yoshizawa, K., Nozaki, S., Kato, A., Hirai, M., Yanase, M., Yoshimoto, T. ... Kawashiri, S. (2013). Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma. Oncology Reports, 29, 445-450. https://doi.org/10.3892/or.2012.2161
MLA
Yoshizawa, K., Nozaki, S., Kato, A., Hirai, M., Yanase, M., Yoshimoto, T., Kimura, I., Sugiura, S., Okamune, A., Kitahara, H., Noguchi, N., Kato, K., Ueki, K., Kawashiri, S."Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma". Oncology Reports 29.2 (2013): 445-450.
Chicago
Yoshizawa, K., Nozaki, S., Kato, A., Hirai, M., Yanase, M., Yoshimoto, T., Kimura, I., Sugiura, S., Okamune, A., Kitahara, H., Noguchi, N., Kato, K., Ueki, K., Kawashiri, S."Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma". Oncology Reports 29, no. 2 (2013): 445-450. https://doi.org/10.3892/or.2012.2161
Copy and paste a formatted citation
x
Spandidos Publications style
Yoshizawa K, Nozaki S, Kato A, Hirai M, Yanase M, Yoshimoto T, Kimura I, Sugiura S, Okamune A, Kitahara H, Kitahara H, et al: Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma. Oncol Rep 29: 445-450, 2013.
APA
Yoshizawa, K., Nozaki, S., Kato, A., Hirai, M., Yanase, M., Yoshimoto, T. ... Kawashiri, S. (2013). Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma. Oncology Reports, 29, 445-450. https://doi.org/10.3892/or.2012.2161
MLA
Yoshizawa, K., Nozaki, S., Kato, A., Hirai, M., Yanase, M., Yoshimoto, T., Kimura, I., Sugiura, S., Okamune, A., Kitahara, H., Noguchi, N., Kato, K., Ueki, K., Kawashiri, S."Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma". Oncology Reports 29.2 (2013): 445-450.
Chicago
Yoshizawa, K., Nozaki, S., Kato, A., Hirai, M., Yanase, M., Yoshimoto, T., Kimura, I., Sugiura, S., Okamune, A., Kitahara, H., Noguchi, N., Kato, K., Ueki, K., Kawashiri, S."Loss of claudin-7 is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma". Oncology Reports 29, no. 2 (2013): 445-450. https://doi.org/10.3892/or.2012.2161
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